دورية أكاديمية
Fatty acid synthase drives the synthesis of phospholipids partitioning into detergent-resistant membrane microdomains.
| العنوان: | Fatty acid synthase drives the synthesis of phospholipids partitioning into detergent-resistant membrane microdomains. |
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| المؤلفون: | Swinnen JV; Laboratory for Experimental Medicine and Endocrinology (LEGENDO), University of Leuven, Gasthuisberg, Herestraat 49, B-3000 Leuven, Belgium. Johan.Swinnen@med.kuleuven.ac.be, Van Veldhoven PP, Timmermans L, De Schrijver E, Brusselmans K, Vanderhoydonc F, Van de Sande T, Heemers H, Heyns W, Verhoeven G |
| المصدر: | Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2003 Mar 21; Vol. 302 (4), pp. 898-903. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: United States NLM ID: 0372516 Publication Model: Print Cited Medium: Print ISSN: 0006-291X (Print) Linking ISSN: 0006291X NLM ISO Abbreviation: Biochem Biophys Res Commun Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: <2002- >: San Diego, CA : Elsevier Original Publication: New York, Academic Press. |
| مواضيع طبية MeSH: | Detergents/*chemistry , Fatty Acid Synthases/*metabolism , Membrane Microdomains/*metabolism , Phospholipids/*metabolism, Down-Regulation/physiology ; Humans ; Male ; Membrane Microdomains/chemistry ; Prostatic Neoplasms ; RNA, Small Interfering/metabolism ; Tumor Cells, Cultured |
| مستخلص: | Fatty acid synthase (FAS) is a key metabolic enzyme catalyzing the synthesis of long-chain saturated fatty acids. It plays a central role in the production of surfactant in fetal lungs, in the supply of fatty components of milk, and in the conversion and storage of energy in liver and adipose tissue. Remarkably high levels of FAS expression are found in the majority of human epithelial cancers. As the role of FAS in cancer cells remains largely unknown, we have initiated studies to assess the fate of newly synthesized lipids in cancer cells and have estimated the contribution of FAS to the synthesis of specific lipid classes by treating the cells with small interfering RNAs targeting FAS. Here, we show that in cancer cells FAS plays a major role in the synthesis of phospholipids partitioning into detergent-resistant membrane microdomains. These are raft-aggregates implicated in key cellular processes including signal transduction, intracellular trafficking, cell polarization, and cell migration. These findings reveal a novel role for FAS, provide important new insights into the otherwise poorly understood mechanisms underlying the control of lipid composition of membrane microdomains, and point to a link between FAS overexpression and dysregulation of membrane composition and functioning in tumor cells. |
| المشرفين على المادة: | 0 (Detergents) 0 (Phospholipids) 0 (RNA, Small Interfering) EC 2.3.1.85 (Fatty Acid Synthases) |
| تواريخ الأحداث: | Date Created: 20030321 Date Completed: 20030513 Latest Revision: 20190612 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1016/s0006-291x(03)00265-1 |
| PMID: | 12646257 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 0006-291X |
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| DOI: | 10.1016/s0006-291x(03)00265-1 |