دورية أكاديمية
Regulation of inducible nitricoxide synthase gene transcription by p38 mitogen-activated protein kinase in human embryonic kidney 293 cells.
| العنوان: | Regulation of inducible nitricoxide synthase gene transcription by p38 mitogen-activated protein kinase in human embryonic kidney 293 cells. |
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| المؤلفون: | Guo AH; Key Laboratory for Shock and Microcirculation of PLA, First Military Medical University, Guangzhou 510515, China., Gong XW, Kan WH, Qin QH, Deng P, Wang JZ, Jiang Y |
| المصدر: | Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA [Di Yi Jun Yi Da Xue Xue Bao] 2003 Mar; Vol. 23 (3), pp. 206-9. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Di 1 jun yi da xue Country of Publication: China NLM ID: 9426110 Publication Model: Print Cited Medium: Print ISSN: 1000-2588 (Print) Linking ISSN: 10002588 NLM ISO Abbreviation: Di Yi Jun Yi Da Xue Xue Bao Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Guangzhou Shi Tonghe Zhen : Di 1 jun yi da xue, -2005. |
| مواضيع طبية MeSH: | Gene Expression Regulation, Enzymologic* , Transcription, Genetic*, Mitogen-Activated Protein Kinases/*physiology , Nitric Oxide Synthase/*genetics, Cells, Cultured ; Embryo, Mammalian ; Humans ; Kidney/cytology ; Nitric Oxide Synthase Type II ; Transfection ; p38 Mitogen-Activated Protein Kinases |
| مستخلص: | Objective: To study the transcriptional regulation of inducible nitric oxide synthase (iNOS) gene by p38 mitogen-activated protein kinase (MAPK). Methods: With human embryonic kidney (HEK) 293 cells as the target and the assistance of lipofectamine-mediated co-transfection techniques and luciferase reporter gene systems, FLAG-tagged p38 isoforms (namely FLAG-p38 alpha, FLAG-p38 beta;, FLAG-p38 gamma and FLAG-p38 phi;) in pcDNA3, pcDNA3, piNOS-Luc and pCMV-beta; were transfected into HEK 293 cells, and the relative activity of luciferase was subsequently tested. Results: Highest luciferase activity occurred only in p38 alpha group compared with the other three isoform groups under no stimulation. Under the stimulation by lipopolysaccharide (LPS), the luciferase activity of each group was obviously increased and the highest activity occurred in p38 beta group. Conclusion: LPS can induce transcription and activation of iNOS gene, and p38 MAPK is involved in the transcription regulation of iNOS gene in HEK 293 cells. |
| المشرفين على المادة: | EC 1.14.13.39 (NOS2 protein, human) EC 1.14.13.39 (Nitric Oxide Synthase) EC 1.14.13.39 (Nitric Oxide Synthase Type II) EC 2.7.11.24 (Mitogen-Activated Protein Kinases) EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases) |
| تواريخ الأحداث: | Date Created: 20030326 Date Completed: 20031215 Latest Revision: 20111027 |
| رمز التحديث: | 20231215 |
| PMID: | 12651229 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1000-2588 |
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