دورية أكاديمية
[Regulation of human oct-1 gene transcription involves two promotors].
| العنوان: | [Regulation of human oct-1 gene transcription involves two promotors]. |
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| عنوان ترانسليتريتد: | Reguliatsiia transkriptsii gena oct-1 cheloveka s uchastiem dvukh promotorov. |
| المؤلفون: | Zhenilo SV; Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991 Russia., Deev IE, Serov SM, Polianovskiĭ OL |
| المصدر: | Genetika [Genetika] 2003 Feb; Vol. 39 (2), pp. 280-5. |
| نوع المنشور: | English Abstract; Journal Article |
| اللغة: | Russian |
| بيانات الدورية: | Publisher: Izdatelstvo Nauka Country of Publication: Russia (Federation) NLM ID: 0047354 Publication Model: Print Cited Medium: Print ISSN: 0016-6758 (Print) Linking ISSN: 00166758 NLM ISO Abbreviation: Genetika Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Moskva : Izdatelstvo Nauka |
| مواضيع طبية MeSH: | Gene Expression Regulation* , Promoter Regions, Genetic* , Transcription, Genetic*, DNA-Binding Proteins/*genetics , Transcription Factors/*genetics, 5' Flanking Region ; Base Composition ; Cells, Cultured ; Cloning, Molecular ; DNA-Binding Proteins/metabolism ; Enhancer Elements, Genetic ; Exons ; Host Cell Factor C1 ; Humans ; Lymphocytes/physiology ; Octamer Transcription Factor-1 ; Transcription Factors/metabolism |
| مستخلص: | Transcription initiation of human Oct-1 transcription factor-encoding gene involves two promoters, 1U and 1L, located at a substantial distance (about 100 kb) apart. The structure of these promoters and the adjacent sequences is different. Specifically, the 1U sequence is GC-rich, while the 1L sequence is AT-rich. Correspondingly, more than 25 GC-rich Sp1 cis-elements were localized within the 1U region, while in the 1L sequence nearly equal amount of homeo-specific NTAATNN sites along with two ATGCAAAT octamers were found. Analysis of transfection of recombinant plasmids, carrying the promoter fragments with or without enhancer indicated that expression from the 1L promoter was tissue-specific. In nonlymphoid HEK293 cells efficiency of transcription from the 1U promoter was several times higher than that from the 1L promoter. Another expression pattern was observed at transfection of the same constructs into Raji lymphoid cells. In this case the level of transcription from the L promoter (fragment L2) at the presence of external enhancer was higher than that from the fragments containing the 1U promoter. It was shown that the distal regions of 1U and 1L were capable of silencing activity. In Raji cells enhancer completely overcomes the activity of U silencer, but only partly overcomes the activity of L silencer. Our data on the interaction of two promoters with the enhancer and silencer in different cell types point to fine tissue-specific regulation of the oct-1 gene expression, especially in lymphatic cells. |
| المشرفين على المادة: | 0 (DNA-Binding Proteins) 0 (HCFC1 protein, human) 0 (Host Cell Factor C1) 0 (Octamer Transcription Factor-1) 0 (POU2F1 protein, human) 0 (Transcription Factors) |
| تواريخ الأحداث: | Date Created: 20030403 Date Completed: 20030606 Latest Revision: 20081121 |
| رمز التحديث: | 20240627 |
| PMID: | 12669425 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 0016-6758 |
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