دورية أكاديمية
Alteration in myelin-associated proteins following spinal cord irradiation in guinea pigs.
| العنوان: | Alteration in myelin-associated proteins following spinal cord irradiation in guinea pigs. |
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| المؤلفون: | Chiang CS; Department of Radiation Oncology, UCLA Medical Center 90024-1714., Mason KA, Withers HR, McBride WH |
| المصدر: | International journal of radiation oncology, biology, physics [Int J Radiat Oncol Biol Phys] 1992; Vol. 24 (5), pp. 929-37. |
| نوع المنشور: | Journal Article; Research Support, U.S. Gov't, P.H.S. |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier, Inc Country of Publication: United States NLM ID: 7603616 Publication Model: Print Cited Medium: Print ISSN: 0360-3016 (Print) Linking ISSN: 03603016 NLM ISO Abbreviation: Int J Radiat Oncol Biol Phys Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: New York, NY : Elsevier, Inc Original Publication: Elmsford, N. Y., Pergamon Press. |
| مواضيع طبية MeSH: | Phosphoric Diester Hydrolases*, 2',3'-Cyclic-Nucleotide Phosphodiesterases/*analysis , Myelin Basic Protein/*analysis , Spinal Cord/*radiation effects, 2',3'-Cyclic Nucleotide 3'-Phosphodiesterase ; Animals ; Dose-Response Relationship, Radiation ; Female ; Guinea Pigs ; Myelin Basic Protein/cerebrospinal fluid ; Paralysis/etiology ; Paralysis/metabolism ; Spinal Cord/chemistry ; Spinal Cord/pathology ; Time Factors |
| مستخلص: | The aim of this study was to investigate the pathological and cellular basis for radiation-induced myelopathy in guinea pigs by monitoring biochemical alterations in levels of myelin basic protein and 2',3'-cyclic nucleotide phosphohydrolase. Guinea pigs were irradiated to the lumbar region with various doses of neutrons or cobalt gamma irradiation. The ED50s for paralysis were 17.2 Gy and 67.5 Gy for neutron and cobalt irradiation, respectively, and was histologically associated with demyelination. In spinal cords taken from animals at the onset of paralysis myelin basic protein levels were decreased in direct relationship to the radiation dose. The lowest doses to cause paralysis led to a 25% decrease in MBP levels. In a separate experiment, alterations in MBP were measured in the spinal cords over the time period leading up to paralysis. Surprisingly, decreases in MBP were found immediately after the end of the 4 week irradiation period. These early changes in MBP were not markedly dose dependent and occurred with nonparalyzing doses. Dose-dependent decreases were found only just before the onset of paralysis. CNPase activity measured in the same specimens showed changes that were essentially similar to those for MBP. In the CSF, MBP levels were essentially constant until onset of paralysis. This study showed that demyelination, as assessed by the levels of the myelin-associated proteins MBP and CNPase, can occur soon after spinal cord irradiation but that profound dose-dependent changes are seen only immediately preceding the onset of paralysis. Although increases in MBP in the CSF were associated with the onset of radiation-induced myelopathy, its assay is unlikely to predict this complication of irradiation. |
| التعليقات: | Comment in: Int J Radiat Oncol Biol Phys. 1995 Feb 1;31(3):689-90. (PMID: 7531678) |
| معلومات مُعتمدة: | CA-44384 United States CA NCI NIH HHS; CA-44881 United States CA NCI NIH HHS |
| المشرفين على المادة: | 0 (Myelin Basic Protein) EC 3.1.4.- (2',3'-Cyclic-Nucleotide Phosphodiesterases) EC 3.1.4.- (Phosphoric Diester Hydrolases) EC 3.1.4.37 (2',3'-Cyclic Nucleotide 3'-Phosphodiesterase) |
| تواريخ الأحداث: | Date Created: 19920101 Date Completed: 19921229 Latest Revision: 20190708 |
| رمز التحديث: | 20221208 |
| DOI: | 10.1016/0360-3016(92)90477-y |
| PMID: | 1280252 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 0360-3016 |
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| DOI: | 10.1016/0360-3016(92)90477-y |