دورية أكاديمية
[Construction of vector of his-tagged cytoplasmic fragment of human toll like receptor 4 and its expression in E.coli].
| العنوان: | [Construction of vector of his-tagged cytoplasmic fragment of human toll like receptor 4 and its expression in E.coli]. |
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| المؤلفون: | Liu JH; Department of Pathophysiology and Key Laboratory of Shock and Microcirculation of People's Liberation Army, First Military Medical University, Guangzhou 510515, Guangdong, China., Sun XG, Li ZJ, Qin QH, Gong XW, Deng P, Liu YW, Wang JZ, Zhao KS, Jiang Y |
| المصدر: | Zhongguo wei zhong bing ji jiu yi xue = Chinese critical care medicine = Zhongguo weizhongbing jijiuyixue [Zhongguo Wei Zhong Bing Ji Jiu Yi Xue] 2003 Mar; Vol. 15 (3), pp. 163-6. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | Chinese |
| بيانات الدورية: | Publisher: Zhonghua yi xue za zhi she Country of Publication: China NLM ID: 9887521 Publication Model: Print Cited Medium: Print ISSN: 1003-0603 (Print) Linking ISSN: 10030603 NLM ISO Abbreviation: Zhongguo Wei Zhong Bing Ji Jiu Yi Xue Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Beijing : Zhonghua yi xue za zhi she Original Publication: Tianjin : Zhongguo wei zhong bing ji jiu yi xue za zhi bian ji bu |
| مواضيع طبية MeSH: | Escherichia coli/*genetics , Genetic Vectors/*genetics , Membrane Glycoproteins/*genetics , Receptors, Cell Surface/*genetics, Cloning, Molecular/methods ; Electrophoresis, Polyacrylamide Gel ; Humans ; Polymerase Chain Reaction ; Recombinant Fusion Proteins/genetics ; Recombinant Fusion Proteins/metabolism ; Toll-Like Receptor 4 ; Toll-Like Receptors |
| مستخلص: | Objective: To construction of vector of his-tagged cytoplasmic fragment of human Toll like receptor 4 (hTLR4) and its expression in E.coli. Methods: hTLR4 cytoplasmic cDNA codon domain was amplified by polymerase chain reaction (PCR) and cloned into pET-DsbA2.0 plasmid expressing His-DsbA fusion protein. After being identified by the assay of restrictional enzyme and sequencing, His-Dsb A fusion proteins were induced with isopropy-beta-D-thiogalactoside (IPTG) and further purified. Results: A fusion protein with molecular weight of 42 kd was obtained. Conclusion: hTLR4 which was constructed and expressed successfully under nondenaturing conditions provides a tool for further studies. |
| المشرفين على المادة: | 0 (Membrane Glycoproteins) 0 (Receptors, Cell Surface) 0 (Recombinant Fusion Proteins) 0 (TLR4 protein, human) 0 (Toll-Like Receptor 4) 0 (Toll-Like Receptors) |
| تواريخ الأحداث: | Date Created: 20030702 Date Completed: 20040623 Latest Revision: 20201208 |
| رمز التحديث: | 20240829 |
| PMID: | 12831622 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1003-0603 |
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