دورية أكاديمية
On the redox equilibrium between H2 and hydrogenase.
| العنوان: | On the redox equilibrium between H2 and hydrogenase. |
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| المؤلفون: | Coremans JM; E.C. Slater Institute for Biochemical Research, University of Amsterdam, The Netherlands., van Garderen CJ, Albracht SP |
| المصدر: | Biochimica et biophysica acta [Biochim Biophys Acta] 1992 Feb 26; Vol. 1119 (2), pp. 148-56. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Pub. Co Country of Publication: Netherlands NLM ID: 0217513 Publication Model: Print Cited Medium: Print ISSN: 0006-3002 (Print) Linking ISSN: 00063002 NLM ISO Abbreviation: Biochim Biophys Acta Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Amsterdam : Elsevier Pub. Co. |
| مواضيع طبية MeSH: | Hydrogen/*chemistry , Hydrogenase/*chemistry , Nickel/*chemistry, Binding Sites ; Chromatium/enzymology ; Electron Spin Resonance Spectroscopy ; Hydrogen-Ion Concentration ; Methanobacterium/enzymology ; Oxidation-Reduction ; Temperature |
| مستخلص: | Redox titrations of the nickel ion in active hydrogenase from Methanobacterium thermoautotrophicum and Chromatium vinosum were performed in the absence of artificial redox mediators, by variation of the H2-partial pressure. These experiments revealed a redox behaviour of the nickel ion which differed remarkably from previous redox titrations in the presence of redox mediators. Notably the EPR signal of the species earlier characterized as monovalent nickel with bound hydrogen, behaved as an n = 2 redox component upon reduction under varying H2-partial pressures. The EPR signal was not a transient one and persisted upon removal of hydrogen. Possible redox processes to explain these observations are discussed. A similar behaviour of nickel was also observed in enzyme as present in intact cells of M. thermoautotrophicum. These results suggest that nickel hydrogenases possess a second site for reaction with H2. |
| المشرفين على المادة: | 7OV03QG267 (Nickel) 7YNJ3PO35Z (Hydrogen) EC 1.12.7.2 (Hydrogenase) |
| تواريخ الأحداث: | Date Created: 19920226 Date Completed: 19920406 Latest Revision: 20190609 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1016/0167-4838(92)90385-q |
| PMID: | 1311607 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 0006-3002 |
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| DOI: | 10.1016/0167-4838(92)90385-q |