التفاصيل البيبلوغرافية
| العنوان: |
Age-related changes in the transcriptional profile of mouse RPE/choroid. |
| المؤلفون: |
Ida H; Department of Biological Chemistry, University of California, Davis, California 95616-8794, USA., Boylan SA, Weigel AL, Hjelmeland LM |
| المصدر: |
Physiological genomics [Physiol Genomics] 2003 Nov 11; Vol. 15 (3), pp. 258-62. Date of Electronic Publication: 2003 Nov 11. |
| نوع المنشور: |
Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.; Validation Study |
| اللغة: |
English |
| بيانات الدورية: |
Publisher: American Physiological Society Country of Publication: United States NLM ID: 9815683 Publication Model: Electronic Cited Medium: Internet ISSN: 1531-2267 (Electronic) Linking ISSN: 10948341 NLM ISO Abbreviation: Physiol Genomics Subsets: MEDLINE |
| أسماء مطبوعة: |
Original Publication: Bethesda, MD : American Physiological Society, c1999- |
| مواضيع طبية MeSH: |
Transcription, Genetic*, Aging/*genetics , Choroid/*metabolism , Gene Expression Profiling/*methods , Pigment Epithelium of Eye/*metabolism , Retina/*metabolism, Animals ; Bruch Membrane/chemistry ; Bruch Membrane/metabolism ; Choroid/chemistry ; DNA, Complementary/genetics ; Gene Expression Profiling/statistics & numerical data ; Gene Expression Regulation ; Male ; Mice ; Mice, Inbred C57BL ; Oligonucleotide Array Sequence Analysis/methods ; Oligonucleotide Array Sequence Analysis/statistics & numerical data ; Pigment Epithelium of Eye/chemistry ; Retina/chemistry ; Reverse Transcriptase Polymerase Chain Reaction/methods ; Reverse Transcriptase Polymerase Chain Reaction/statistics & numerical data |
| مستخلص: |
To evaluate the age-related changes in gene expression occurring in the complex of retinal pigmented epithelium, Bruch's membrane, and choroid (RPE/choroid), we examined the gene expression profiles of young adult (2 mo) and old (24 mo) male C57BL/6 mice. cDNA probe sets from individual animals were synthesized using total RNA isolated from the RPE/choroid of each animal. Probes were amplified using the Clontech SMART system, radioactively labeled, and hybridized to two different Clontech Atlas mouse cDNA arrays. From each age group, three independent triplicates were hybridized to the arrays. Statistical analyses were performed using the Significance Analysis of Microarrays program (SAM version 1.13; Stanford University). Selected array results were confirmed by semi-quantitative RT-PCR analysis. Of 2,340 genes represented on the arrays, approximately 60% were expressed in young and/or old mouse RPE/choroid. A moderate fraction (12%) of all expressed genes exhibited a statistically significant change in expression with age. Of these 150 genes, all but two, HMG14 and carboxypeptidase E, were upregulated with age. Many of these upregulated genes can be grouped into several broad functional categories: immune response, proteases and protease inhibitors, stress response, and neovascularization. RT-PCR results from six of six genes examined confirmed the differential change in expression with age of these genes. Our study provides likely candidate genes to further study their role in the development of age-related macular degeneration and other aging diseases affecting the RPE/choroid. |
| معلومات مُعتمدة: |
EY-06473 United States EY NEI NIH HHS; P30-EY-12576 United States EY NEI NIH HHS |
| المشرفين على المادة: |
0 (DNA, Complementary) |
| تواريخ الأحداث: |
Date Created: 20031002 Date Completed: 20031216 Latest Revision: 20191210 |
| رمز التحديث: |
20240628 |
| DOI: |
10.1152/physiolgenomics.00126.2003 |
| PMID: |
14519767 |
| قاعدة البيانات: |
MEDLINE |
