دورية أكاديمية
Establishment and characterization of novel xenograft models of human biliary tract carcinomas.
| العنوان: | Establishment and characterization of novel xenograft models of human biliary tract carcinomas. |
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| المؤلفون: | Emura F; Department of Surgery, Institute of Clinical Medicine, University of Tsukuba, Tsukuba-shi, Ibaraki 305-8575, Japan., Kamma H, Ghosh M, Koike N, Kawamoto T, Saijo K, Ohno T, Ohkohchi N, Todoroki T |
| المصدر: | International journal of oncology [Int J Oncol] 2003 Nov; Vol. 23 (5), pp. 1293-300. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: D.A. Spandidos Country of Publication: Greece NLM ID: 9306042 Publication Model: Print Cited Medium: Print ISSN: 1019-6439 (Print) Linking ISSN: 10196439 NLM ISO Abbreviation: Int J Oncol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: <2003->: Athens, Greece : D.A. Spandidos Original Publication: Athens, Greece : Lychnia, |
| مواضيع طبية MeSH: | Disease Models, Animal*, Biliary Tract Neoplasms/*pathology , Carcinoma/*pathology, Animals ; Blotting, Western ; Cell Line, Tumor ; Humans ; Immunohistochemistry ; Karyotyping ; Male ; Mice ; Mice, Nude ; Neoplasm Transplantation ; Prognosis ; Species Specificity ; Time Factors |
| مستخلص: | In order to develop new therapeutic regimens for biliary tract cancers, which carry dismal prognoses, the establishment of a human biliary tract cancer xenograft model is essential. Herein, we report the successful establishment and characterization of two xenograft models of human biliary tract cancers. An adenosquamous gallbladder cancer cell line (TGBC-44) and a bile duct adenocarcinoma cell line (TGBC-47) were obtained from fresh surgical specimens in our department and subcutaneously inoculated into nude mice. The overall tumor take rate was 100% and solid tumors grew measurable after 5 and 7 days for TGBC-44 and TGBC-47, respectively. Tumor doubling time was 3.9+/-1.1 and 4.1+/-0.5 days in the exponential growth phase in TGBC-44 and TGBC-47 xenografts, respectively. Isozyme test and karyotype analysis confirmed the human origin. Histopathology analysis revealed that the TGBC-44 xenograft retained both the squamous and the adenocarcinoma components, and the TGBC-47 xenograft exhibited poorly differentiated adenocarcinoma as in the corresponding original tumors. Immunohistochemistry and Western blotting studies revealed positive and similar expression of platelet derived endothelial growth factor/thymidine phosphorylase (PDGF/TP), thymidylate synthase (TS), and cyclooxygenase-2 (COX-2) in both original tumors and xenograft models. No macroscopic metastases were found at the time of sacrifice. We have successfully established two models of human biliary tract cancer, gallbladder and bile duct cancer. Models retained the morphological and biochemical characteristics of the original tumor and demonstrated constant biological behavior in all transplanted mice. These models could be useful tools for developing new diagnostic and therapeutic strategies against biliary tract cancers. |
| تواريخ الأحداث: | Date Created: 20031009 Date Completed: 20040726 Latest Revision: 20220409 |
| رمز التحديث: | 20221213 |
| PMID: | 14532968 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1019-6439 |
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