دورية أكاديمية
Survival and differentiation of adult rat-derived neural progenitor cells transplanted to the striatum of hemiparkinsonian rats.
| العنوان: | Survival and differentiation of adult rat-derived neural progenitor cells transplanted to the striatum of hemiparkinsonian rats. |
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| المؤلفون: | Dziewczapolski G; Department of Neurosciences, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093-0662, USA., Lie DC, Ray J, Gage FH, Shults CW |
| المصدر: | Experimental neurology [Exp Neurol] 2003 Oct; Vol. 183 (2), pp. 653-64. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. |
| اللغة: | English |
| بيانات الدورية: | Publisher: Academic Press Country of Publication: United States NLM ID: 0370712 Publication Model: Print Cited Medium: Print ISSN: 0014-4886 (Print) Linking ISSN: 00144886 NLM ISO Abbreviation: Exp Neurol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Orlando Fl : Academic Press Original Publication: New York. |
| مواضيع طبية MeSH: | Corpus Striatum/*pathology , Neurons/*cytology , Parkinsonian Disorders/*therapy , Stem Cell Transplantation/*methods , Stem Cells/*cytology, Animals ; Antigens/biosynthesis ; Cell Differentiation ; Cell Movement ; Cell Survival ; Corpus Striatum/cytology ; Disease Models, Animal ; Female ; Genes, Reporter ; Graft Survival ; Green Fluorescent Proteins ; Hippocampus/cytology ; Luminescent Proteins/biosynthesis ; Luminescent Proteins/genetics ; Neurons/drug effects ; Oxidopamine ; Parkinsonian Disorders/chemically induced ; Parkinsonian Disorders/pathology ; Proteoglycans/biosynthesis ; Rats ; Rats, Inbred F344 ; Stem Cells/metabolism |
| مستخلص: | We investigated the survival, distribution and differentiation capabilities of adult rat hippocampus-derived progenitor cells (AHPs) by grafting them into either the intact or dopamine (DA)-denervated adult rat striatum (ST). Furthermore, we tested the effects of the in vivo administration of retinoic acid (RA) on the differentiation of the grafted cells. AHPs, prelabeled in vitro with bromodeoxyuridine (BrdU) and primed with RA, were transplanted bilaterally into the ST of hemiparkinsonian rats. Twenty animals were divided in four groups: three groups received i.p. injections of RA (1.5 mg/kg/day) for 1, 2 or 4 weeks and one group received vehicle injections for 4 weeks. Approximately 60% of the implanted BrdU-immunoreactive (BrdU+) cells were present in either intact or lesioned ST after 5 weeks of transplantation, with a striking widespread radial distribution from the implantation site. The cells became morphologically integrated with the surrounding host tissue, with no evidence of tumor formation. Approximately 18% of the BrdU+ cells were immunoreactive for the glial precursor marker NG2 and occasionally BrdU+ cells co-expressed the neuronal marker TuJ1. This differentiation pattern was similar in the intact and DA-denervated ST. Although further research is needed to find more adequate methods to drive the differentiation of these cells toward the desired phenotypes, the survival, differentiation potential and widespread distribution throughout the ST observed in this study suggest that AHPs may be useful in treatment of degenerative disorders affecting the nervous system. |
| المشرفين على المادة: | 0 (Antigens) 0 (Luminescent Proteins) 0 (Proteoglycans) 0 (chondroitin sulfate proteoglycan 4) 147336-22-9 (Green Fluorescent Proteins) 8HW4YBZ748 (Oxidopamine) |
| تواريخ الأحداث: | Date Created: 20031014 Date Completed: 20031031 Latest Revision: 20190722 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1016/s0014-4886(03)00212-7 |
| PMID: | 14552907 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 0014-4886 |
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| DOI: | 10.1016/s0014-4886(03)00212-7 |