تقرير
Amplified voltammetric detection of DNA hybridization via oxidation of ferrocene caps on gold nanoparticle/streptavidin conjugates.
| العنوان: | Amplified voltammetric detection of DNA hybridization via oxidation of ferrocene caps on gold nanoparticle/streptavidin conjugates. |
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| المؤلفون: | Wang J, Li J, Baca AJ, Hu J, Zhou F, Yan W, Pang DW |
| المصدر: | Analytical chemistry [Anal Chem] 2003 Aug 01; Vol. 75 (15), pp. 3941-5. |
| نوع المنشور: | Letter; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Chemical Society Country of Publication: United States NLM ID: 0370536 Publication Model: Print Cited Medium: Print ISSN: 0003-2700 (Print) Linking ISSN: 00032700 NLM ISO Abbreviation: Anal Chem Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Washington, American Chemical Society. |
| مواضيع طبية MeSH: | DNA/*analysis , DNA/*chemistry , Electrochemistry/*methods , Ferrous Compounds/*chemistry , Gold/*chemistry , Nucleic Acid Hybridization/*methods , Streptavidin/*chemistry, DNA, Viral/blood ; DNA, Viral/genetics ; Elasticity ; Electrodes ; Electron Transport ; Hepatitis B virus/genetics ; Metallocenes ; Nanotechnology ; Oxidation-Reduction ; Reproducibility of Results ; Sensitivity and Specificity |
| مستخلص: | Gold nanoparticle/streptavidin conjugates covered with 6-ferrocenylhexanethiol were attached onto a biotinylated DNA detection probe of a sandwich DNA complex. Due to the elasticity of the DNA strands, the ferrocene caps on gold nanoparticle/streptavidin conjugates are positioned in close proximity to the underlying electrode modified with a mixed DNA capture probe/hexanethiol self-assembled monolayer and can undergo reversible electron-transfer reactions. A detection level, down to 2.0 pM (10 amol for the 5 microL of sample needed) for oligodeoxynucleotide samples was obtained. The amplification of the voltammetric signals was attributed to the attachment of a large number of redox (ferrocene) markers per DNA duplex formed. The ferrocene oxidation current increased with the target concentration and began to level off at a target concentration of 10 nM. An Excellent linearity was found within the range between 6.9 and 150.0 pM and reasonable relative standard deviations (between 3.0 and 13.0%) were obtained. The amenability of this method to the analyses of polynucleotides (i.e., PCR products of the pre-S gene of hepatitis B virus in serum samples) was also demonstrated. The method is shown to be simple, selective, reproducible, and cost-effective and does not require labeling of the DNA targets. |
| معلومات مُعتمدة: | GM 08101 United States GM NIGMS NIH HHS |
| المشرفين على المادة: | 0 (DNA, Viral) 0 (Ferrous Compounds) 0 (Metallocenes) 7440-57-5 (Gold) 9007-49-2 (DNA) 9013-20-1 (Streptavidin) U96PKG90JQ (ferrocene) |
| تواريخ الأحداث: | Date Created: 20031024 Date Completed: 20040719 Latest Revision: 20190717 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1021/ac0344079 |
| PMID: | 14572067 |
| قاعدة البيانات: | MEDLINE |
Find this issue from the American Chemical Society | تدمد: | 0003-2700 |
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| DOI: | 10.1021/ac0344079 |