دورية أكاديمية
Genome-wide analysis of DNA methylation status of CpG islands in embryoid bodies, teratomas, and fetuses.
| العنوان: | Genome-wide analysis of DNA methylation status of CpG islands in embryoid bodies, teratomas, and fetuses. |
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| المؤلفون: | Kremenskoy M; Laboratory of Cellular Biochemistry, Animal Resource Science/Veterinary Medical Sciences, Graduate School of Agriculture and Life Science, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, 113-8657, Tokyo, Japan., Kremenska Y, Ohgane J, Hattori N, Tanaka S, Hashizume K, Shiota K |
| المصدر: | Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2003 Nov 28; Vol. 311 (4), pp. 884-90. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: United States NLM ID: 0372516 Publication Model: Print Cited Medium: Print ISSN: 0006-291X (Print) Linking ISSN: 0006291X NLM ISO Abbreviation: Biochem Biophys Res Commun Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: <2002- >: San Diego, CA : Elsevier Original Publication: New York, Academic Press. |
| مواضيع طبية MeSH: | DNA Methylation*, Cell Differentiation/*genetics , Chromosome Mapping/*methods , CpG Islands/*genetics , Restriction Mapping/*methods , Stem Cells/*physiology , Teratoma/*genetics, Animals ; Brain/cytology ; Female ; Fetus/cytology ; Gene Expression Profiling/methods ; Kidney/cytology ; Male ; Mice ; Mice, Inbred C57BL ; Stem Cells/cytology ; Teratoma/pathology |
| مستخلص: | Differentiation of embryonic stem (ES) cells into embryoid bodies (EBs) provides an in vitro system for the study of early lineage determination during mammalian development. We have previously reported that there are 247 CpG islands that potentially have tissue-dependent and differentially methylated regions (T-DMRs). This provided evidence that the formation of DNA methylation patterns at CpG islands is a crucial epigenetic event underlying mammalian development. Here we present an analysis by the restriction landmark genomic scanning (RLGS) using NotI as a landmark enzyme of the genome-wide methylation status of CpG islands of ES cells and EBs and of teratomas produced from ES cells. These results are considered in relation to the methylation status of CpG islands of genomic DNA from normal fetus (10.5dpc) and adult tissues. We have prepared a DNA methylation panel that consists of 259 T-DMRs and includes novel T-DMRs that are distinctly methylated or unmethylated in the teratomas. The DNA methylation pattern was complex and differed for the ES cells, EBs, and teratomas, providing evidence that differentiation of cells involves both de novo DNA methylation as well as demethylation. Comparison of the numbers of T-DMRs, that were differentially methylated or unmethylated among the cells and tissue types studied, revealed that the teratomas were the most epigenetically different from ES cells. Thus, analysis of the DNA methylation profiles prepared in this study provides new insights into the differentiation of ES cells and development of fetus, EB, teratoma, and somatic tissues. |
| تواريخ الأحداث: | Date Created: 20031119 Date Completed: 20040209 Latest Revision: 20190612 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1016/j.bbrc.2003.10.078 |
| PMID: | 14623263 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 0006-291X |
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| DOI: | 10.1016/j.bbrc.2003.10.078 |