دورية أكاديمية
[Screening and identification of proteins interacting with p38 MAP kinase via T7 phage-display screening system].
| العنوان: | [Screening and identification of proteins interacting with p38 MAP kinase via T7 phage-display screening system]. |
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| المؤلفون: | Li ZJ; Department of Pathophysiology and Key Laboratory of Shock and Microcirculation of PLA, First Military Medical University, Guangzhou 510515, China., Liu JH, Gong XW, Qin QH, Huang H, Deng P, Wang JZ, Zhao SC, Sun XG, Liu YW, Zhao KS, Jiang Y |
| المصدر: | Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA [Di Yi Jun Yi Da Xue Xue Bao] 2003 Nov; Vol. 23 (11), pp. 1131-3. |
| نوع المنشور: | English Abstract; Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | Chinese |
| بيانات الدورية: | Publisher: Di 1 jun yi da xue Country of Publication: China NLM ID: 9426110 Publication Model: Print Cited Medium: Print ISSN: 1000-2588 (Print) Linking ISSN: 10002588 NLM ISO Abbreviation: Di Yi Jun Yi Da Xue Xue Bao Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Guangzhou Shi Tonghe Zhen : Di 1 jun yi da xue, -2005. |
| مواضيع طبية MeSH: | Bacteriophage T7/*genetics , Mitogen-Activated Protein Kinases/*metabolism, Gene Library ; Humans ; Protein Binding ; p38 Mitogen-Activated Protein Kinases |
| مستخلص: | Objective: To screen and identify proteins that interact with p38 mitogen-activated protein (MAP) kinases by means of T7 phage-display screening system. Method: His-tagged fusion protein of p38 MAP kinase was used to coat a 96-well ELISA plate and Ni-NTA resin, which served as the media for screening human lung and liver T7 phage cDNA libraries. Results: After 4 rounds of biopanning, 86 independent plaques were selected and processed by EDTA. The inserted gene fragments from these plaques were amplified by PCR, the products purified by a gel recovery method. The sequences of the insertions were identified and analyzed with BLAST program in GenBank. Forty-six clones were found to encode proteins. Conclusion: T7 phage-display screening system is convenient, rapid and effective for screening the P38 MAP kinase-binding proteins. |
| المشرفين على المادة: | EC 2.7.11.24 (Mitogen-Activated Protein Kinases) EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases) |
| تواريخ الأحداث: | Date Created: 20031120 Date Completed: 20040331 Latest Revision: 20091119 |
| رمز التحديث: | 20221213 |
| PMID: | 14625169 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1000-2588 |
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