دورية أكاديمية
A cohort study of telomere and telomerase biology in cats.
| العنوان: | A cohort study of telomere and telomerase biology in cats. |
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| المؤلفون: | McKevitt TP; Institute of Comparative Medicine, Department of Veterinary Clinical Studies, Glasgow University Veterinary School, Glasgow University, Glasgow, UK G61 1QH., Nasir L, Wallis CV, Argyle DJ |
| المصدر: | American journal of veterinary research [Am J Vet Res] 2003 Dec; Vol. 64 (12), pp. 1496-9. |
| نوع المنشور: | Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Veterinary Medical Association Country of Publication: United States NLM ID: 0375011 Publication Model: Print Cited Medium: Print ISSN: 0002-9645 (Print) Linking ISSN: 00029645 NLM ISO Abbreviation: Am J Vet Res Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Schaumburg, Ill. : American Veterinary Medical Association Original Publication: Chicago : American Veterinary Medical Assn. |
| مواضيع طبية MeSH: | Aging/*genetics , Cats/*genetics , Cats/*physiology , Telomerase/*physiology , Telomere/*genetics, Age Factors ; Animals ; Autoradiography ; Cohort Studies ; Polymorphism, Restriction Fragment Length |
| مستخلص: | Objective: To investigate telomere lengths in tissues of domestic shorthair (DSH) cats of various ages, evaluate the relationship between telomere length and age of cats, and investigate telomerase activity in the somatic tissues of cats. Sample Population: Tissues obtained from 2 DSH cats and blood samples obtained from 30 DSH cats. Procedure: DNA isolated from blood cells and somatic tissue samples was subjected to terminal restriction fragment (TRF) analysis to determine mean telomere repeat lengths. Protein samples were subjected to analysis by use of a telomeric repeat-amplification protocol to assess telomerase activity. Results: MeanTRF values of cats ranged from 4.7 to 26.3 kilobase pairs, and there was significant telomeric attrition with increasing age of cat. Telomerase activity was not found in a wide range of normal tissues obtained from 2 cats. Conclusions and Clinical Relevance: Analysis of these results clearly indicates that telomeres are shorter in older cats, compared with young cats; therefore, telomeres are implicated in the aging process. The analysis of telomerase activity in normal somatic tissues of cats reveals a pattern of expression similar to that found in human tissues. Impact for Human Medicine: Fundamental differences in the biological characteristics of telomeres and telomerase exist between humans and the other most widely studied species (ie, mice). The results reported here reveal similarities in telomere and telomerase biologic characteristics between DSH cats and humans. Hence, as well as developing our understanding of aging in cats, these data may be usefully extrapolated to aging in humans. |
| المشرفين على المادة: | EC 2.7.7.49 (Telomerase) |
| تواريخ الأحداث: | Date Created: 20031216 Date Completed: 20040105 Latest Revision: 20190917 |
| رمز التحديث: | 20231215 |
| DOI: | 10.2460/ajvr.2003.64.1496 |
| PMID: | 14672427 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 0002-9645 |
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| DOI: | 10.2460/ajvr.2003.64.1496 |