دورية أكاديمية
The B subunit of the CCAAT box binding transcription factor complex (CBF/NF-Y) is essential for early mouse development and cell proliferation.
| العنوان: | The B subunit of the CCAAT box binding transcription factor complex (CBF/NF-Y) is essential for early mouse development and cell proliferation. |
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| المؤلفون: | Bhattacharya A; Department of Molecular Genetics, University of Texas MD Anderson Cancer Center, Houston, Texas 77030, USA., Deng JM, Zhang Z, Behringer R, de Crombrugghe B, Maity SN |
| المصدر: | Cancer research [Cancer Res] 2003 Dec 01; Vol. 63 (23), pp. 8167-72. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Association for Cancer Research Country of Publication: United States NLM ID: 2984705R Publication Model: Print Cited Medium: Print ISSN: 0008-5472 (Print) Linking ISSN: 00085472 NLM ISO Abbreviation: Cancer Res Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Baltimore, Md. : American Association for Cancer Research Original Publication: Chicago [etc.] |
| مواضيع طبية MeSH: | CCAAT-Binding Factor/*physiology , Mice/*embryology, Alleles ; Animals ; Apoptosis/genetics ; Apoptosis/physiology ; CCAAT-Binding Factor/genetics ; CCAAT-Binding Factor/metabolism ; Cell Cycle/genetics ; Cell Cycle/physiology ; Cell Division/genetics ; Cell Division/physiology ; Gene Deletion ; Mice/genetics ; Mice, Inbred C57BL ; S Phase/genetics ; S Phase/physiology ; Transcription, Genetic |
| مستخلص: | To understand the physiological function of the mammalian heterotrimeric CCAAT binding factor CBF, also known as NF-Y, we have generated a conditional Cbf-b mouse mutant by introducing loxP sites in the murine Cbf-b/Nf-ya gene. Controlled expression of Cre recombinase deletes the gene in vivo, which leads to a loss of DNA binding by the CBF complex and hence CBF-mediated transcription. Deletion of both Cbf-b alleles causes early embryo lethality, indicating that CBF activity is essential for early mouse development. In primary cultures of mouse embryonic fibroblasts, conditional inactivation of CBF results in a block in cell proliferation and inhibition of S phase or DNA synthesis, which is followed by induction of apoptosis. We conclude that the CBF transcription factor complex is essential for cell proliferation and viability. |
| المشرفين على المادة: | 0 (CCAAT-Binding Factor) |
| تواريخ الأحداث: | Date Created: 20031218 Date Completed: 20040227 Latest Revision: 20031217 |
| رمز التحديث: | 20221213 |
| PMID: | 14678971 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 0008-5472 |
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