دورية أكاديمية
ICAM-3 activation modulates cell-cell contacts of human bone marrow endothelial cells.
| العنوان: | ICAM-3 activation modulates cell-cell contacts of human bone marrow endothelial cells. |
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| المؤلفون: | van Buul JD; Department of Experimental Immunohematology, Sanquin Research at CLB and the Landsteiner Laboratorium, University of Amsterdam, Amsterdam, The Netherlands., Mul FP, van der Schoot CE, Hordijk PL |
| المصدر: | Journal of vascular research [J Vasc Res] 2004 Jan-Feb; Vol. 41 (1), pp. 28-37. Date of Electronic Publication: 2004 Jan 12. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: S. Karger Country of Publication: Switzerland NLM ID: 9206092 Publication Model: Print-Electronic Cited Medium: Print ISSN: 1018-1172 (Print) Linking ISSN: 10181172 NLM ISO Abbreviation: J Vasc Res Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Basel ; New York : S. Karger, c1992- |
| مواضيع طبية MeSH: | Antigens, CD/*metabolism , Antigens, Differentiation/*metabolism , Bone Marrow Cells/*cytology , Cell Communication/*physiology , Endothelium, Vascular/*cytology, Bone Marrow Cells/metabolism ; Cell Adhesion Molecules ; Cells, Cultured ; Cytoskeletal Proteins ; Electric Impedance ; Endothelium, Vascular/metabolism ; Flow Cytometry ; Humans ; Microfilament Proteins/metabolism ; Phosphoproteins/metabolism ; Reactive Oxygen Species/metabolism ; T-Lymphocytes/cytology ; T-Lymphocytes/metabolism ; Umbilical Veins/cytology |
| مستخلص: | The Ig-like cell adhesion molecule ICAM-3 is mainly expressed on human leukocytes and is involved in cell-cell interactions. Its expression on endothelium is observed during disorders such as Crohn's disease and in solid tumors. We found low but detectable expression of ICAM-3 on VE-cadherin-expressing cells from primary human bone marrow aspirates, i.e. endothelial cells, and on primary human endothelial cells from cord blood. Also, immortalized human umbilical cord endothelial cells and human bone marrow endothelial cells showed ICAM-3 expression. However, its function on human endothelium is not known. Surprisingly, activation of endothelial ICAM-3 by crosslinking with specific antibodies resulted in a drop in the electrical resistance of bone marrow endothelial monolayers. In line with this, immunocytochemical analysis showed a loss of endothelial cell-cell contacts after ICAM-3 crosslinking in HBMEC. Detailed biochemical analysis showed an association of moesin and in a later stage ezrin with ICAM-3 upon crosslinking in HBMEC. Moreover, ICAM-3 crosslinking induced the production of reactive oxygen species (ROS), which are known to be involved in the control of endothelial cell-cell contacts. In conclusion, we showed that ICAM-3 is expressed on human bone marrow endothelial cells and controls endothelial integrity via ROS-dependent signaling. (Copyright 2004 S. Karger AG, Basel) |
| المشرفين على المادة: | 0 (Antigens, CD) 0 (Antigens, Differentiation) 0 (Cell Adhesion Molecules) 0 (Cytoskeletal Proteins) 0 (ICAM3 protein, human) 0 (Microfilament Proteins) 0 (Phosphoproteins) 0 (Reactive Oxygen Species) 0 (ezrin) 144131-77-1 (moesin) |
| تواريخ الأحداث: | Date Created: 20040117 Date Completed: 20040323 Latest Revision: 20220227 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1159/000076126 |
| PMID: | 14726630 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1018-1172 |
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| DOI: | 10.1159/000076126 |