دورية أكاديمية
أعرض في EDS

Reciprocal regulation of thymus and activation-regulated chemokine/macrophage-derived chemokine production by interleukin (IL)-4/IL-13 and interferon-gamma in HaCaT keratinocytes is mediated by alternations in E-cadherin distribution.

التفاصيل البيبلوغرافية
العنوان: Reciprocal regulation of thymus and activation-regulated chemokine/macrophage-derived chemokine production by interleukin (IL)-4/IL-13 and interferon-gamma in HaCaT keratinocytes is mediated by alternations in E-cadherin distribution.
المؤلفون: Fujii-Maeda S; Clinical Research Center for Allergy, National Sagamihara Hospital, Sagamihara, Kanagawa, 228-8522, Japan., Kajiwara K, Ikizawa K, Shinazawa M, Yu B, Koga T, Furue M, Yanagihara Y
المصدر: The Journal of investigative dermatology [J Invest Dermatol] 2004 Jan; Vol. 122 (1), pp. 20-8.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Elsevier Country of Publication: United States NLM ID: 0426720 Publication Model: Print Cited Medium: Print ISSN: 0022-202X (Print) Linking ISSN: 0022202X NLM ISO Abbreviation: J Invest Dermatol Subsets: MEDLINE
أسماء مطبوعة: Publication: 2016- : New York : Elsevier
Original Publication: Baltimore, Williams & Wilkins.
مواضيع طبية MeSH: Cadherins/*metabolism , Chemokines, CC/*metabolism , Cytokines/*pharmacology , Keratinocytes/*metabolism, Adherens Junctions/drug effects ; Adherens Junctions/metabolism ; Antineoplastic Agents/pharmacology ; Cell Adhesion/physiology ; Cell Line ; Chemokine CCL17 ; Chemokine CCL22 ; Chemokines, CC/genetics ; Down-Regulation/drug effects ; Down-Regulation/immunology ; Gene Expression/drug effects ; Gene Expression/immunology ; Humans ; Interferon-gamma/pharmacology ; Interleukin-13/pharmacology ; Interleukin-4/pharmacology ; Keratinocytes/immunology ; RNA, Messenger/analysis ; Up-Regulation/drug effects ; Up-Regulation/immunology
مستخلص: Keratinocytes produce many cytochemokines that are involved in the pathogenesis of skin disorders. In particular, the CC chemokines thymus and activation-regulated chemokine (TARC)/macrophage-derived chemokine (MDC) play an important role in the infiltration of Th2 cells. This study was undertaken to examine the regulatory effects of interleukin (IL)-4, IL-13, and interferon (IFN)-gamma on TARC/MDC production in the human keratinocyte cell line HaCaT. HaCaT cells spontaneously secrete TARC and MDC. The production of TARC/MDC was downregulated by IL-4/IL-13, whereas it was upregulated by IFN-gamma. To explore these regulatory mechanisms, we investigated the capacity of cytokines to regulate expression of several adhesion molecules that may affect TARC/MDC production. Of the adhesion molecules examined, the constitutive surface expression of E-cadherin was downregulated by IL-4/IL-13, but was upregulated by IFN-gamma. Moreover, disruption of the homophilic adherence of E-cadherin by anti-E-cadherin antibody or calcium chelation abolished the production of TARC/MDC. We further examined the distribution of the adherens junction complex composed of E-cadherin, alpha-catenin, beta-catenin, and gamma-catenin. IL-4/IL-13 decreased the levels of membrane staining for adherens junction proteins, whereas IFN-gamma increased membrane staining. Taken together, these results suggest that IL-4/IL-13 and IFN-gamma induce alternations in the distribution of adherens junctions in a different fashion and thereby contribute to the reciprocal regulation of TARC/MDC production.
المشرفين على المادة: 0 (Antineoplastic Agents)
0 (CCL17 protein, human)
0 (CCL22 protein, human)
0 (Cadherins)
0 (Chemokine CCL17)
0 (Chemokine CCL22)
0 (Chemokines, CC)
0 (Cytokines)
0 (Interleukin-13)
0 (RNA, Messenger)
207137-56-2 (Interleukin-4)
82115-62-6 (Interferon-gamma)
تواريخ الأحداث: Date Created: 20040214 Date Completed: 20040304 Latest Revision: 20181130
رمز التحديث: 20221213
DOI: 10.1046/j.0022-202X.2003.22103.x
PMID: 14962085
قاعدة البيانات: MEDLINE
الوصف
تدمد:0022-202X
DOI:10.1046/j.0022-202X.2003.22103.x