دورية أكاديمية
Reciprocal regulation of thymus and activation-regulated chemokine/macrophage-derived chemokine production by interleukin (IL)-4/IL-13 and interferon-gamma in HaCaT keratinocytes is mediated by alternations in E-cadherin distribution.
العنوان: | Reciprocal regulation of thymus and activation-regulated chemokine/macrophage-derived chemokine production by interleukin (IL)-4/IL-13 and interferon-gamma in HaCaT keratinocytes is mediated by alternations in E-cadherin distribution. |
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المؤلفون: | Fujii-Maeda S; Clinical Research Center for Allergy, National Sagamihara Hospital, Sagamihara, Kanagawa, 228-8522, Japan., Kajiwara K, Ikizawa K, Shinazawa M, Yu B, Koga T, Furue M, Yanagihara Y |
المصدر: | The Journal of investigative dermatology [J Invest Dermatol] 2004 Jan; Vol. 122 (1), pp. 20-8. |
نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
اللغة: | English |
بيانات الدورية: | Publisher: Elsevier Country of Publication: United States NLM ID: 0426720 Publication Model: Print Cited Medium: Print ISSN: 0022-202X (Print) Linking ISSN: 0022202X NLM ISO Abbreviation: J Invest Dermatol Subsets: MEDLINE |
أسماء مطبوعة: | Publication: 2016- : New York : Elsevier Original Publication: Baltimore, Williams & Wilkins. |
مواضيع طبية MeSH: | Cadherins/*metabolism , Chemokines, CC/*metabolism , Cytokines/*pharmacology , Keratinocytes/*metabolism, Adherens Junctions/drug effects ; Adherens Junctions/metabolism ; Antineoplastic Agents/pharmacology ; Cell Adhesion/physiology ; Cell Line ; Chemokine CCL17 ; Chemokine CCL22 ; Chemokines, CC/genetics ; Down-Regulation/drug effects ; Down-Regulation/immunology ; Gene Expression/drug effects ; Gene Expression/immunology ; Humans ; Interferon-gamma/pharmacology ; Interleukin-13/pharmacology ; Interleukin-4/pharmacology ; Keratinocytes/immunology ; RNA, Messenger/analysis ; Up-Regulation/drug effects ; Up-Regulation/immunology |
مستخلص: | Keratinocytes produce many cytochemokines that are involved in the pathogenesis of skin disorders. In particular, the CC chemokines thymus and activation-regulated chemokine (TARC)/macrophage-derived chemokine (MDC) play an important role in the infiltration of Th2 cells. This study was undertaken to examine the regulatory effects of interleukin (IL)-4, IL-13, and interferon (IFN)-gamma on TARC/MDC production in the human keratinocyte cell line HaCaT. HaCaT cells spontaneously secrete TARC and MDC. The production of TARC/MDC was downregulated by IL-4/IL-13, whereas it was upregulated by IFN-gamma. To explore these regulatory mechanisms, we investigated the capacity of cytokines to regulate expression of several adhesion molecules that may affect TARC/MDC production. Of the adhesion molecules examined, the constitutive surface expression of E-cadherin was downregulated by IL-4/IL-13, but was upregulated by IFN-gamma. Moreover, disruption of the homophilic adherence of E-cadherin by anti-E-cadherin antibody or calcium chelation abolished the production of TARC/MDC. We further examined the distribution of the adherens junction complex composed of E-cadherin, alpha-catenin, beta-catenin, and gamma-catenin. IL-4/IL-13 decreased the levels of membrane staining for adherens junction proteins, whereas IFN-gamma increased membrane staining. Taken together, these results suggest that IL-4/IL-13 and IFN-gamma induce alternations in the distribution of adherens junctions in a different fashion and thereby contribute to the reciprocal regulation of TARC/MDC production. |
المشرفين على المادة: | 0 (Antineoplastic Agents) 0 (CCL17 protein, human) 0 (CCL22 protein, human) 0 (Cadherins) 0 (Chemokine CCL17) 0 (Chemokine CCL22) 0 (Chemokines, CC) 0 (Cytokines) 0 (Interleukin-13) 0 (RNA, Messenger) 207137-56-2 (Interleukin-4) 82115-62-6 (Interferon-gamma) |
تواريخ الأحداث: | Date Created: 20040214 Date Completed: 20040304 Latest Revision: 20181130 |
رمز التحديث: | 20221213 |
DOI: | 10.1046/j.0022-202X.2003.22103.x |
PMID: | 14962085 |
قاعدة البيانات: | MEDLINE |
تدمد: | 0022-202X |
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DOI: | 10.1046/j.0022-202X.2003.22103.x |