دورية أكاديمية
In vitro and in vivo models for peritonitis demonstrate unchanged neutrophil migration after exposure to dialysis fluids.
| العنوان: | In vitro and in vivo models for peritonitis demonstrate unchanged neutrophil migration after exposure to dialysis fluids. |
|---|---|
| المؤلفون: | Welten AG; Department of Molecular Cell Biology and Immunology, VU University Medical Center, FdG, Postbus 7057, 1007 MB Amsterdam, The Netherlands. j.vandenborn@vumc.nl, Zareie M, van den Born J, ter Wee PM, Schalkwijk CG, Driesprong BA, Mul FP, Hordijk PL, Beelen RH, Hekking LH |
| المصدر: | Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association [Nephrol Dial Transplant] 2004 Apr; Vol. 19 (4), pp. 831-9. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Oxford University Press Country of Publication: England NLM ID: 8706402 Publication Model: Print Cited Medium: Print ISSN: 0931-0509 (Print) Linking ISSN: 09310509 NLM ISO Abbreviation: Nephrol Dial Transplant Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Oxford : Oxford University Press Original Publication: [Berlin ; New York, NY] : Springer International, [c1986- |
| مواضيع طبية MeSH: | Disease Models, Animal* , Peritoneal Dialysis*, Neutrophils/*physiology , Peritonitis/*immunology , Peritonitis/*microbiology, Body Fluids ; Cell Movement ; Cells, Cultured ; Epithelial Cells ; Escherichia coli Infections/microbiology ; Humans ; Peritoneum/cytology ; Peritoneum/microbiology ; Staphylococcal Infections/microbiology |
| مستخلص: | Background: Recurrent infections in peritoneal dialysis (PD) patients may alter the abdominal wall resulting in an impairment of its dialysis capacity. In this study we investigated both in vitro and in vivo the effects of mesothelial exposure to dialysis fluids on the migration of neutrophils and their capacity to clear a bacterial infection. Methods: First, we evaluated neutrophil migration in an in vitro transwell model for the peritoneal membrane with monolayers of primary human mesothelial cells (MC) on the lower side and primary human endothelial cells (EC) on top of the same transwell membrane, upon exposure of MC to PD fluid (PDF)-derived components. In addition to this in vitro model, we combined chronic peritoneal exposure to PDF with a peritoneal infection model in the rat. We investigated the kinetics of the chemokine response, neutrophil recruitment and bacterial clearance. Results: Known chemoattractants, such as fMLP and IL-8, strongly increased neutrophil migration across both cell layers in the in vitro model of the peritoneal membrane. Pre-incubation of the MC layer for 48 h with 55 mM glucose, a combination of two glucose degradation products, methylglyoxal and 3-deoxyglucosone, or conventional dialysis fluid (1:4 dilution), however, did not change the IL-8-induced migration of neutrophils. In concert with this finding we demonstrated an unchanged MC expression of ICAM-1 and VCAM-1 after these pre-treatments. Unexpectedly, chronic i.p. exposure to conventional PDF or a recently developed lactate/bicarbonate-buffered PDF in a rat peritoneal exposure model strongly hampered the chemokine response upon bacterial challenge. Nevertheless, neutrophil recruitment and bacterial clearance were effective and did not differ from rats not pre-exposed to PDF. Conclusions: We conclude that exposure of MC to PDF does not hamper the recruitment of functional neutrophils upon challenge. |
| تواريخ الأحداث: | Date Created: 20040320 Date Completed: 20041007 Latest Revision: 20061115 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1093/ndt/gfh024 |
| PMID: | 15031337 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 0931-0509 |
|---|---|
| DOI: | 10.1093/ndt/gfh024 |