دورية أكاديمية
Poly(L-lysine)-modified silica nanoparticles for the delivery of antisense oligonucleotides.
| العنوان: | Poly(L-lysine)-modified silica nanoparticles for the delivery of antisense oligonucleotides. |
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| المؤلفون: | Zhu SG; Cancer Research Institute, XiangYa School of Medicine, Central South University, ChangSha, HuNan 410078, People's Republic of China., Xiang JJ, Li XL, Shen SR, Lu HB, Zhou J, Xiong W, Zhang BC, Nie XM, Zhou M, Tang K, Li GY |
| المصدر: | Biotechnology and applied biochemistry [Biotechnol Appl Biochem] 2004 Apr; Vol. 39 (Pt 2), pp. 179-87. |
| نوع المنشور: | Evaluation Study; Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Wiley-Blackwell Country of Publication: United States NLM ID: 8609465 Publication Model: Print Cited Medium: Print ISSN: 0885-4513 (Print) Linking ISSN: 08854513 NLM ISO Abbreviation: Biotechnol Appl Biochem Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Jan. 2011- : Malden : Wiley-Blackwell Original Publication: San Diego : Academic Press, [cl986]- |
| مواضيع طبية MeSH: | Carcinoma/*genetics , Drug Carriers/*chemistry , Nanotubes/*chemistry , Oligoribonucleotides, Antisense/*administration & dosage , Polylysine/*chemistry , Silicon Dioxide/*chemistry , Transfection/*methods, Carcinoma/metabolism ; Carcinoma/pathology ; Cell Line, Tumor ; Coated Materials, Biocompatible/chemistry ; Gene Transfer Techniques ; HeLa Cells ; Humans ; Materials Testing ; Nanotubes/ultrastructure ; Oligoribonucleotides, Antisense/chemistry ; Oligoribonucleotides, Antisense/genetics ; Oligoribonucleotides, Antisense/pharmacokinetics ; Particle Size ; Surface Properties ; Tissue Distribution |
| مستخلص: | Silica nanoparticles were prepared in a microemulsion system, using polyoxyethylene nonylphenyl ether/cyclohexane/ammonium hydroxide. The surface charge of the particle was modified with PLL [poly(L-lysine)]. PAGE demonstrated the ability of PMS-NP (PLL-modified silica nanoparticles) to bind and protect antisense ODNs (oligonucleotides). The intracellular localization of FITC-labelled ODN was investigated by fluorescence microscopy. The results demonstrated that ODN could be delivered to cytoplasm. Flow-cytometry analysis showed a 20-fold enhancement of ODN delivered by PMS-NP compared with free ODN for a serum-free medium. Blocking efficacy of c- myc antisense ODN, delivered by PMS-NP, was examined in HNE1 and HeLa cell lines. Significant down-regulation of c- myc mRNA levels was observed in both the cell lines. However, the cellular uptake efficiency and antisense effects on target gene decreased in the presence of serum-containing medium. The analysis of the filtration assay showed that PMS-NP interacted with serum proteins. These results indicated that PMS-NP was a suitable delivery vector for antisense ODN, although its delivery efficiency decreased in the presence of a serum-containing medium. |
| المشرفين على المادة: | 0 (Coated Materials, Biocompatible) 0 (Drug Carriers) 0 (Oligoribonucleotides, Antisense) 25104-18-1 (Polylysine) 7631-86-9 (Silicon Dioxide) |
| تواريخ الأحداث: | Date Created: 20040323 Date Completed: 20041220 Latest Revision: 20191210 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1042/BA20030077 |
| PMID: | 15032738 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 0885-4513 |
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| DOI: | 10.1042/BA20030077 |