التفاصيل البيبلوغرافية
| العنوان: |
[Microchips based on three dimensional gel cells: history and perspective]. |
| عنوان ترانسليتريتد: |
Mikrochipy na osnove trekhmernykh iacheek gelia: istoriia i perspektivy. |
| المؤلفون: |
Kolchinskiĭ AM; Health Front Line, Ltd., Champaign, IL, USA., Griadunov DA, Lysov IuP, Mikhaĭlovich VM, Nasedkina TV, Turygin AIu, Rubina AIu, Barskiĭ VE, Zasedatelev AS |
| المصدر: |
Molekuliarnaia biologiia [Mol Biol (Mosk)] 2004 Jan-Feb; Vol. 38 (1), pp. 5-16. |
| نوع المنشور: |
English Abstract; Journal Article; Review |
| اللغة: |
Russian |
| بيانات الدورية: |
Publisher: Izdatelstvo Nauka Country of Publication: Russia (Federation) NLM ID: 0105454 Publication Model: Print Cited Medium: Print ISSN: 0026-8984 (Print) Linking ISSN: 00268984 NLM ISO Abbreviation: Mol Biol (Mosk) Subsets: MEDLINE |
| أسماء مطبوعة: |
Original Publication: Moskva : Izdatelstvo Nauka |
| مواضيع طبية MeSH: |
Gels* , Semiconductors*, Genomics ; Proteomics |
| مستخلص: |
The review describes the history of creation and development of the microchip technology and its role in the human genome project in Russia. The emphasis is placed on the three-dimensional gel-based microchips developed at the Center of Biological Microchips headed by A.D. Mirzabekov since 1988. The gel-based chips of the last generation, IMAGE chips (Immobilized Micro Array of Gel Elements), have a number of advantages over the previous versions. The microchips are manufactured by photo-initiated copolymerization of gel components and immobilized molecules (DNA, proteins, and ligands). This ensures an even distribution of the immobilized probe throughout the microchip gel element with a high yield (about 50% for oligonucleotides). The use of methacrylamide as a main component of the polymerization mixture resulted in a substantial increase of gel porosity without affecting its mechanical strength and stability, which allowed one to work with the DNA fragments of up to 500 nt in length, as well as with rather large protein molecules. At present, the gel-based microchips are widely applied to address different problems. The generic microchips containing a complete set of possible hexanucleotides are used to reveal the DNA motifs binding with different proteins and to study the DNA-protein interactions. The oligonucleotide microchips are a cheap and reliable tool of diagnostics designed for mass application. Biochips have been developed for identification of the tuberculosis pathogen and its antibiotic-resistant forms; for diagnostics of orthopoxviruses, including the smallpox virus; for diagnostics of the anthrax pathogen; and for identification of chromosomal rearrangements in leukemia patients. The protein microchips can be adapted for further use in proteomics. Bacterial and yeast cells were also immobilized in the gel, maintaining their viability, which open a wide potential for creation biosensors on the basis of microchips. |
| Number of References: |
43 |
| المشرفين على المادة: |
0 (Gels) |
| تواريخ الأحداث: |
Date Created: 20040327 Date Completed: 20040713 Latest Revision: 20061115 |
| رمز التحديث: |
20221213 |
| PMID: |
15042831 |
| قاعدة البيانات: |
MEDLINE |
