دورية أكاديمية
أعرض في EDS

Initiation of cytokinesis is controlled through multiple modes of regulation of the Sid2p-Mob1p kinase complex.

التفاصيل البيبلوغرافية
العنوان: Initiation of cytokinesis is controlled through multiple modes of regulation of the Sid2p-Mob1p kinase complex.
المؤلفون: Hou MC; Department of Molecular Genetics and Microbiology and Program in Cell Dynamics, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA., Guertin DA, McCollum D
المصدر: Molecular and cellular biology [Mol Cell Biol] 2004 Apr; Vol. 24 (8), pp. 3262-76.
نوع المنشور: Journal Article; Research Support, U.S. Gov't, P.H.S.
اللغة: English
بيانات الدورية: Publisher: Taylor & Francis Country of Publication: United States NLM ID: 8109087 Publication Model: Print Cited Medium: Print ISSN: 0270-7306 (Print) Linking ISSN: 02707306 NLM ISO Abbreviation: Mol Cell Biol Subsets: MEDLINE
أسماء مطبوعة: Publication: 2023- : [Philadelphia] : Taylor & Francis
Original Publication: [Washington, D.C.] : American Society for Microbiology, [c1981-
مواضيع طبية MeSH: DNA-Binding Proteins*, Cell Cycle Proteins/*metabolism , Cell Division/*physiology , Phosphoproteins/*metabolism , Protein Kinases/*metabolism , Proteins/*metabolism , Saccharomyces cerevisiae Proteins/*metabolism , Schizosaccharomyces pombe Proteins/*metabolism, Amino Acid Sequence ; Animals ; Cell Cycle/physiology ; Cell Cycle Proteins/genetics ; Enzyme Activation ; Macromolecular Substances ; Molecular Sequence Data ; Phosphoproteins/genetics ; Phosphorylation ; Protein Binding ; Protein Kinases/genetics ; Protein Structure, Tertiary ; Proteins/genetics ; Saccharomyces cerevisiae Proteins/genetics ; Schizosaccharomyces/cytology ; Schizosaccharomyces/enzymology ; Schizosaccharomyces/physiology ; Schizosaccharomyces pombe Proteins/genetics ; Sequence Alignment ; Signal Transduction/physiology
مستخلص: The Sid2p-Mob1p kinase complex is an important component of the septation initiation network (SIN) in the fission yeast Schizosaccharomyces pombe. However, regulation of this complex is still elusive. Here we show that Mob1p is required not only for the subcellular localization of Sid2p but also for its kinase activity. We identified a region at the amino terminus of Sid2p that is required for Mob1p binding and spindle pole body (SPB) localization. Deletion of this region abolishes Mob1p binding and diminishes SPB localization, whereas this region alone is sufficient to associate with Mob1p and SPBs. We further show that a similar region of the N terminus of the Sid2p-related protein kinase Orb6p binds to the Mob1p-related protein Mob2p, suggesting that this may be a conserved mode of interaction for this family of kinases. Phosphorylation of Ser402 and especially Thr578 is important for Sid2p function. Sid2p with a mutation of Thr578 to Ala (T578A) can no longer rescue sid2-250 mutant cells, and this results in reduction of Mob1p binding. Sid2p mutants mimicking phosphorylation at this site (T578D and T578E) can rescue sid2-250 cells, enhance Sid2p kinase activity, and partially rescue growth defects of upstream sin mutants. Interestingly, Sid2p, but not Mob1p, is self-associated. Our experiments suggest that self-associated Sid2p is inactive. This self-association is mediated by a region that overlaps with Mob1p and SPB binding sites. Overexpression of Mob1p is able to disrupt the self-association of Sid2p. Taken together, our results suggest that Sid2p kinase may utilize multiple modes of regulation including self-association, Mob1p binding, and phosphorylation to achieve its full activity at an appropriate time and place in the cell.
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معلومات مُعتمدة: R01 GM058406 United States GM NIGMS NIH HHS; GM58406 United States GM NIGMS NIH HHS
المشرفين على المادة: 0 (Cell Cycle Proteins)
0 (DNA-Binding Proteins)
0 (MOB1 protein, S cerevisiae)
0 (Macromolecular Substances)
0 (Phosphoproteins)
0 (Proteins)
0 (Saccharomyces cerevisiae Proteins)
0 (Schizosaccharomyces pombe Proteins)
0 (TAH11 protein, S cerevisiae)
0 (cdt1 protein, S pombe)
EC 2.7.- (Protein Kinases)
EC 2.7.1.- (Sid2 protein, S pombe)
تواريخ الأحداث: Date Created: 20040403 Date Completed: 20040519 Latest Revision: 20210526
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC381652
DOI: 10.1128/MCB.24.8.3262-3276.2004
PMID: 15060149
قاعدة البيانات: MEDLINE
الوصف
تدمد:0270-7306
DOI:10.1128/MCB.24.8.3262-3276.2004