دورية أكاديمية
Overexpression of the HER-2 oncogene does not play a role in high-grade osteosarcomas.
| العنوان: | Overexpression of the HER-2 oncogene does not play a role in high-grade osteosarcomas. |
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| المؤلفون: | Anninga JK; Department of Pediatric Hematology, Oncology and Bone Marrow Transplantation, Leiden University Medical Center, J6S, PO Box 9600, 2300 RC Leiden, The Netherlands. j.k.anninga@lumc.nl, van de Vijver MJ, Cleton-Jansen AM, Kristel PM, Taminiau AH, Nooij M, Egeler RM, Hogendoorn PC |
| المصدر: | European journal of cancer (Oxford, England : 1990) [Eur J Cancer] 2004 May; Vol. 40 (7), pp. 963-70. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Science Ltd Country of Publication: England NLM ID: 9005373 Publication Model: Print Cited Medium: Print ISSN: 0959-8049 (Print) Linking ISSN: 09598049 NLM ISO Abbreviation: Eur J Cancer Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Oxford : Elsevier Science Ltd Original Publication: Oxford ; New York : Pergamon Press, c1990- |
| مواضيع طبية MeSH: | Genes, erbB-2*, Bone Neoplasms/*metabolism , Osteosarcoma/*metabolism , Receptor, ErbB-2/*metabolism, Adolescent ; Adult ; Aged ; Aged, 80 and over ; Bone Neoplasms/genetics ; Child ; Gene Amplification ; Humans ; Immunohistochemistry ; Middle Aged ; Osteosarcoma/genetics ; RNA, Messenger/metabolism ; Reverse Transcriptase Polymerase Chain Reaction/methods |
| مستخلص: | The aim of our study was to determine whether or not the tyrosine kinase receptor, HER2 (also known as ErbB2/Her2/neu), is overexpressed in human osteosarcomas (OS). We studied 15 biopsy and 18 resection specimens at the mRNA and protein levels. HER2 status in the OS specimens was assessed by immunohistochemistry (IHC) and quantitative Real-Time Polymerase chain reaction (PCR). In moderately immunopositive cases fluorescent in situ hybridisation (FISH) analysis was used in order to identify any possible gene amplification. 27 samples were evaluable for IHC and only 1 case showed a moderately positive membrane staining. The remaining samples showed no staining or focal cytoplasmic staining (2 samples). In the moderately positive case, FISH analysis showed no HER-2 gene amplification. There was also no overexpression of HER2 mRNA suggesting this sample was a false-positive immunostain. HER2 mRNA expression was present in all samples at a similar level to that in the breast cancer cell line, MCF7, which does not overexpress HER2 and was used as a negative control. In conclusion, this study shows that HER2 mRNA or membranous HER2 protein overexpression is absent in human OS. We noted various inconsistencies in previous published studies, with regard to methodology and the interpretation of the results based on poor methodology. We therefore conclude that the positive data with regard to HER2 overexpression reported in these previous studies is not reliable. Our results suggest that the monoclonal antibody trastuzumab (Herceptin(R)), directed against the HER2-receptor, is not likely to be an effective therapeutic agent in OS. |
| التعليقات: | Comment in: Eur J Cancer. 2004 May;40(7):924-5. (PMID: 15093566) |
| المشرفين على المادة: | 0 (RNA, Messenger) EC 2.7.10.1 (Receptor, ErbB-2) |
| تواريخ الأحداث: | Date Created: 20040420 Date Completed: 20040610 Latest Revision: 20141120 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1016/j.ejca.2003.10.025 |
| PMID: | 15093570 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 0959-8049 |
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| DOI: | 10.1016/j.ejca.2003.10.025 |