دورية أكاديمية
أعرض في EDS

A transcript finishing initiative for closing gaps in the human transcriptome.

التفاصيل البيبلوغرافية
العنوان: A transcript finishing initiative for closing gaps in the human transcriptome.
المؤلفون: Sogayar MC, Camargo AA, Bettoni F, Carraro DM, Pires LC, Parmigiani RB, Ferreira EN, de Sá Moreira E, do Rosário D de O Latorre M, Simpson AJ, Cruz LO, Degaki TL, Festa F, Massirer KB, Sogayar MC, Filho FC, Camargo LP, Cunha MA, De Souza SJ, Faria M Jr, Giuliatti S, Kopp L, de Oliveira PS, Paiva PB, Pereira AA, Pinheiro DG, Puga RD, S de Souza JE, Albuquerque DM, Andrade LE, Baia GS, Briones MR, Cavaleiro-Luna AM, Cerutti JM, Costa FF, Costanzi-Strauss E, Espreafico EM, Ferrasi AC, Ferro ES, Fortes MA, Furchi JR, Giannella-Neto D, Goldman GH, Goldman MH, Gruber A, Guimarães GS, Hackel C, Henrique-Silva F, Kimura ET, Leoni SG, Macedo C, Malnic B, Manzini B CV, Marie SK, Martinez-Rossi NM, Menossi M, Miracca EC, Nagai MA, Nobrega FG, Nobrega MP, Oba-Shinjo SM, Oliveira MK, Orabona GM, Otsuka AY, Paço-Larson ML, Paixão BM, Pandolfi JR, Pardini MI, Passos Bueno MR, Passos GA, Pesquero JB, Pessoa JG, Rahal P, Rainho CA, Reis CP, Ricca TI, Rodrigues V, Rogatto SR, Romano CM, Romeiro JG, Rossi A, Sá RG, Sales MM, Sant'Anna SC, Santarosa PL, Segato F, Silva WA Jr, Silva ID, Silva NP, Soares-Costa A, Sonati MF, Strauss BE, Tajara EH, Valentini SR, Villanova FE, Ward LS, Zanette DL
مؤلفون مشاركون: Ludwig-FAPESP Transcript Finishing Initiative
المصدر: Genome research [Genome Res] 2004 Jul; Vol. 14 (7), pp. 1413-23. Date of Electronic Publication: 2004 Jun 14.
نوع المنشور: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Cold Spring Harbor Laboratory Press Country of Publication: United States NLM ID: 9518021 Publication Model: Print-Electronic Cited Medium: Print ISSN: 1088-9051 (Print) Linking ISSN: 10889051 NLM ISO Abbreviation: Genome Res Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Cold Spring Harbor, N.Y. : Cold Spring Harbor Laboratory Press, c1995-
مواضيع طبية MeSH: Software*, Transcription, Genetic/*genetics, Alternative Splicing/genetics ; Cell Line ; Cell Line, Tumor ; Computational Biology/methods ; Computational Biology/statistics & numerical data ; Consensus Sequence/genetics ; DNA, Neoplasm ; Databases, Genetic/classification ; Expressed Sequence Tags ; Genes/genetics ; Genome, Human ; HeLa Cells/pathology ; Humans ; Molecular Sequence Data ; Open Reading Frames/genetics ; Software Design ; Software Validation ; U937 Cells/pathology
مستخلص: We report the results of a transcript finishing initiative, undertaken for the purpose of identifying and characterizing novel human transcripts, in which RT-PCR was used to bridge gaps between paired EST clusters, mapped against the genomic sequence. Each pair of EST clusters selected for experimental validation was designated a transcript finishing unit (TFU). A total of 489 TFUs were selected for validation, and an overall efficiency of 43.1% was achieved. We generated a total of 59,975 bp of transcribed sequences organized into 432 exons, contributing to the definition of the structure of 211 human transcripts. The structure of several transcripts reported here was confirmed during the course of this project, through the generation of their corresponding full-length cDNA sequences. Nevertheless, for 21% of the validated TFUs, a full-length cDNA sequence is not yet available in public databases, and the structure of 69.2% of these TFUs was not correctly predicted by computer programs. The TF strategy provides a significant contribution to the definition of the complete catalog of human genes and transcripts, because it appears to be particularly useful for identification of low abundance transcripts expressed in a restricted set of tissues as well as for the delineation of gene boundaries and alternatively spliced isoforms.
(Copyright 2004 Cold Spring Harbor Laboratory Press ISSN)
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سلسلة جزيئية: GENBANK CF272536; CF272537; CF272538; CF272539; CF272540; CF272541; CF272542; CF272543; CF272544; CF272545; CF272546; CF272547; CF272548; CF272549; CF272550; CF272551; CF272552; CF272553; CF272554; CF272555; CF272556; CF272557; CF272558; CF272559; CF272560; CF272561; CF272562; CF272563; CF272564; CF272565; CF272566; CF272567; CF272568; CF272569; CF272570; CF272571; CF272572; CF272573; CF272574; CF272575; CF272576; CF272577; CF272578; CF272579; CF272580; CF272581; CF272582; CF272583; CF272584; CF272585; CF272586; CF272587; CF272588; CF272589; CF272590; CF272591; CF272592; CF272593; CF272594; CF272595; CF272596; CF272597; CF272598; CF272599; CF272600; CF272601; CF272602; CF272603; CF272604; CF272605; CF272606; CF272607; CF272608; CF272609; CF272610; CF272611; CF272612; CF272613; CF272614; CF272615; CF272616; CF272617; CF272618; CF272619; CF272620; CF272621; CF272622; CF272623; CF272624; CF272625; CF272626; CF272627; CF272628; CF272629; CF272630; CF272631; CF272632; CF272633; CF272634; CF272635; CF272636; CF272637; CF272638; CF272639; CF272640; CF272641; CF272642; CF272643; CF272644; CF272645; CF272646; CF272647; CF272648; CF272649; CF272650; CF272651; CF272652; CF272653; CF272654; CF272655; CF272656; CF272657; CF272658; CF272659; CF272660; CF272661; CF272662; CF272663; CF272664; CF272665; CF272666; CF272667; CF272668; CF272669; CF272670; CF272671; CF272672; CF272673; CF272674; CF272675; CF272676; CF272677; CF272678; CF272679; CF272680; CF272681; CF272682; CF272683; CF272684; CF272685; CF272686; CF272687; CF272688; CF272689; CF272690; CF272691; CF272692; CF272693; CF272694; CF272695; CF272696; CF272697; CF272698; CF272699; CF272700; CF272701; CF272702; CF272703; CF272704; CF272705; CF272706; CF272707; CF272708; CF272709; CF272710; CF272711; CF272712; CF272713; CF272714; CF272715; CF272716; CF272717; CF272718; CF272719; CF272720; CF272721; CF272722; CF272723; CF272724; CF272725; CF272726; CF272727; CF272728; CF272729; CF272730; CF272731; CF272732; CF272733
المشرفين على المادة: 0 (DNA, Neoplasm)
تواريخ الأحداث: Date Created: 20040616 Date Completed: 20040903 Latest Revision: 20240508
رمز التحديث: 20240508
مُعرف محوري في PubMed: PMC442158
DOI: 10.1101/gr.2111304
PMID: 15197164
قاعدة البيانات: MEDLINE
الوصف
تدمد:1088-9051
DOI:10.1101/gr.2111304