دورية أكاديمية
Differential effects of serum constituents on apoptosis induced by the cyclopentenone prostaglandin 15-deoxy-delta12,14-prostaglandin J2 in WISH epithelial cells.
| العنوان: | Differential effects of serum constituents on apoptosis induced by the cyclopentenone prostaglandin 15-deoxy-delta12,14-prostaglandin J2 in WISH epithelial cells. |
|---|---|
| المؤلفون: | Berry EB; Liggins Institute, Faculty of Medical and Health Sciences, University of Auckland, Private Bag 92019, Auckland, New Zealand. ebe.berry@auckland.ac.nz, Sato TA, Mitchell MD, Stewart Gilmour R, Helliwell RJ |
| المصدر: | Prostaglandins, leukotrienes, and essential fatty acids [Prostaglandins Leukot Essent Fatty Acids] 2004 Sep; Vol. 71 (3), pp. 191-7. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Churchill Livingstone Country of Publication: Scotland NLM ID: 8802730 Publication Model: Print Cited Medium: Print ISSN: 0952-3278 (Print) Linking ISSN: 09523278 NLM ISO Abbreviation: Prostaglandins Leukot Essent Fatty Acids Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Edinburgh ; New York : Churchill Livingstone, c1988- |
| مواضيع طبية MeSH: | Apoptosis/*drug effects , Epithelial Cells/*physiology , Prostaglandin D2/*analogs & derivatives , Prostaglandin D2/*pharmacology , Serum Albumin/*physiology, Apoptosis/physiology ; Cell Line ; Humans ; Inflammation/metabolism ; Intercellular Signaling Peptides and Proteins/pharmacology ; Neoplasms/metabolism ; Prostaglandin D2/metabolism ; Receptor, IGF Type 1/metabolism ; Serum ; Serum Albumin/pharmacology ; Signal Transduction/drug effects |
| مستخلص: | Cyclopentenone prostaglandins, delta12-PGJ2 and 15d-PGJ2, have potent anti-tumour and anti-inflammatory activities, and have been shown to induce apoptosis in amnion-derived WISH cells. In this study, we have investigated the protective effects of serum and its constituents (growth factors and albumin) on delta12-PGJ2 and 15d-PGJ2-induced apoptosis in WISH cells. Serum (0.5% w/v) was protective against both delta12-PGJ2 and 15d-PGJ2-induced apoptosis. This was not due to the presence of serum-derived growth factors (EGF, IGF-1 and IGF-2), since they had no significant effect on 15d-PGJ2-induced cell death. In contrast, IGF-1 partially inhibited etoposide-induced apoptosis, confirming the presence of a functional IGF-1 receptor signalling system. Albumin was identified as the key survival factor in serum, since albumin and delipidated albumin exhibited the same level of protection from 15d-PGJ2-induced apoptosis as serum itself. The potential for serum albumin to regulate the bioactivity of cyclopentenone PGs may be of considerable importance in pathological conditions where roles for cyclopentenone PGs have been identified. |
| المشرفين على المادة: | 0 (15-deoxyprostaglandin J2) 0 (Intercellular Signaling Peptides and Proteins) 0 (Serum Albumin) EC 2.7.10.1 (Receptor, IGF Type 1) RXY07S6CZ2 (Prostaglandin D2) |
| تواريخ الأحداث: | Date Created: 20040716 Date Completed: 20050113 Latest Revision: 20131121 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1016/j.plefa.2004.04.001 |
| PMID: | 15253890 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 0952-3278 |
|---|---|
| DOI: | 10.1016/j.plefa.2004.04.001 |