دورية أكاديمية
Semiquantitative analysis of gene expression in cultured chondrocytes by RT-PCR.
| العنوان: | Semiquantitative analysis of gene expression in cultured chondrocytes by RT-PCR. |
|---|---|
| المؤلفون: | Rolland-Valognes G; Division der Rhumatologie, Institut de Recherches Servier, Suresnes, France. |
| المصدر: | Methods in molecular medicine [Methods Mol Med] 2004; Vol. 100, pp. 69-78. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Humana Press Country of Publication: United States NLM ID: 101123138 Publication Model: Print Cited Medium: Print ISSN: 1543-1894 (Print) Linking ISSN: 15431894 NLM ISO Abbreviation: Methods Mol Med Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Totowa, N.J. : Humana Press, c1996- |
| مواضيع طبية MeSH: | Chondrocytes/*metabolism , Gene Expression/*genetics , Reverse Transcriptase Polymerase Chain Reaction/*methods, Cells, Cultured ; Collagen Type II/genetics ; Collagenases/genetics ; Humans ; Matrix Metalloproteinase 13 ; Matrix Metalloproteinase 3/genetics ; RNA/isolation & purification |
| مستخلص: | Reverse transcriptase-polymerase chain reaction (RT-PCR) is a powerful, sensitive, and rapid method to monitor small amounts of nucleic acids. This is of particular interest for small amounts of cells, as in cartilage. We present here two protocols to isolate total RNA and a protocol to study matrix metalloproteinase and type II collagen gene expression from chondrocytes of human origin. Specific gene expression is revealed on an ethidium bromide-containing agarose gel on an ultraviolet plate and normalized to that of a housekeeping gene. |
| المشرفين على المادة: | 0 (Collagen Type II) 63231-63-0 (RNA) EC 3.4.24.- (Collagenases) EC 3.4.24.- (MMP13 protein, human) EC 3.4.24.- (Matrix Metalloproteinase 13) EC 3.4.24.17 (Matrix Metalloproteinase 3) |
| تواريخ الأحداث: | Date Created: 20040729 Date Completed: 20050131 Latest Revision: 20061115 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1385/1-59259-810-2:069 |
| PMID: | 15280588 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1543-1894 |
|---|---|
| DOI: | 10.1385/1-59259-810-2:069 |