دورية أكاديمية
Pathogenesis of acute viral disease induced in fish by carp interstitial nephritis and gill necrosis virus.
| العنوان: | Pathogenesis of acute viral disease induced in fish by carp interstitial nephritis and gill necrosis virus. |
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| المؤلفون: | Pikarsky E; Department of Pathology, The Hebrew University-Hadassah Medical School, Jerusalem, Israel., Ronen A, Abramowitz J, Levavi-Sivan B, Hutoran M, Shapira Y, Steinitz M, Perelberg A, Soffer D, Kotler M |
| المصدر: | Journal of virology [J Virol] 2004 Sep; Vol. 78 (17), pp. 9544-51. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Society For Microbiology Country of Publication: United States NLM ID: 0113724 Publication Model: Print Cited Medium: Print ISSN: 0022-538X (Print) Linking ISSN: 0022538X NLM ISO Abbreviation: J Virol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Washington Dc : American Society For Microbiology Original Publication: Baltimore, American Society for Microbiology. |
| مواضيع طبية MeSH: | Carps/*virology , Fish Diseases/*virology , Gills/*virology , Nephritis, Interstitial/*veterinary , Nephritis, Interstitial/*virology , Virus Diseases/*veterinary , Virus Diseases/*virology , Viruses/*pathogenicity, Acute Disease ; Animals ; Carps/blood ; Cells, Cultured ; Cloning, Molecular ; Cytopathogenic Effect, Viral ; DNA, Viral/blood ; Fish Diseases/pathology ; Genetic Engineering ; Gills/pathology ; Immunohistochemistry ; Kidney/pathology ; Kidney/virology ; Kinetics ; Microscopy, Electron ; Nephritis, Interstitial/pathology ; Virus Diseases/pathology ; Viruses/genetics ; Viruses/isolation & purification ; Viruses/ultrastructure |
| مستخلص: | A lethal disease of koi and common carp (species Cyprinus carpio) has afflicted many fish farms worldwide since 1998, causing severe financial losses. Morbidity and mortality are restricted to common carp and koi and appear in spring and autumn, when water temperatures are 18 to 28 degrees C. We have isolated the virus causing the disease from sick fish, propagated it in koi fin cell culture, and shown that virus from a single clone causes lethal disease in carp and koi upon infection. Intraperitoneal virus injection or bathing the fish in virus-containing water kills 85 to 100% of the fish within 7 to 21 days. This virus is similar to the previously reported koi herpesvirus; however, it has characteristics inconsistent with the herpesvirus family, and thus we have called it carp interstitial nephritis and gill necrosis virus. We examined the pathobiology of this disease in carp by using immunohistochemistry and PCR. We found large amounts of the virus in the kidneys of sick fish and smaller amounts in liver and brain. A rapid increase in the viral load in the kidneys was detected by using both immunofluorescence and semiquantitative PCR. Histological analyses of fish at various times after infection revealed signs of interstitial nephritis as early as 2 days postinfection, which increased in severity up to 10 days postinfection. There was severe gill disease evidenced by loss of villi with accompanying inflammation in the gill rakers. Minimal focal inflammation was noted in livers and brains. This report describes the etiology and pathology of a recently described viral agent in fish. |
| References: | Dis Aquat Organ. 1999 Jun 23;37(1):13-21. (PMID: 10439899) Vet Microbiol. 2002 Apr 22;86(1-2):69-88. (PMID: 11888691) Dis Aquat Organ. 2002 Mar 11;48(2):101-8. (PMID: 12005231) Vet Pathol. 2002 Mar;39(2):180-9. (PMID: 12009056) J Gen Virol. 2003 Oct;84(Pt 10):2661-7. (PMID: 13679599) Vaccine. 2003 Dec 1;21(32):4677-84. (PMID: 14585675) J Aquat Anim Health. 2000 Mar;12(1):44-57. (PMID: 28880775) Zentralbl Veterinarmed B. 1986 Aug;33(6):471-6. (PMID: 3098001) |
| المشرفين على المادة: | 0 (DNA, Viral) |
| تواريخ الأحداث: | Date Created: 20040817 Date Completed: 20040916 Latest Revision: 20181224 |
| رمز التحديث: | 20231215 |
| مُعرف محوري في PubMed: | PMC506927 |
| DOI: | 10.1128/JVI.78.17.9544-9551.2004 |
| PMID: | 15308746 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 0022-538X |
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| DOI: | 10.1128/JVI.78.17.9544-9551.2004 |