دورية أكاديمية
Susceptibility of skin cells to UVA-induced necrotic cell death reflects the intracellular level of labile iron.
| العنوان: | Susceptibility of skin cells to UVA-induced necrotic cell death reflects the intracellular level of labile iron. |
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| المؤلفون: | Zhong JL; Department of Pharmacy and Pharmacology, University of Bath, Bath, UK., Yiakouvaki A, Holley P, Tyrrell RM, Pourzand C |
| المصدر: | The Journal of investigative dermatology [J Invest Dermatol] 2004 Oct; Vol. 123 (4), pp. 771-80. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: United States NLM ID: 0426720 Publication Model: Print Cited Medium: Print ISSN: 0022-202X (Print) Linking ISSN: 0022202X NLM ISO Abbreviation: J Invest Dermatol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2016- : New York : Elsevier Original Publication: Baltimore, Williams & Wilkins. |
| مواضيع طبية MeSH: | Cell Death/*radiation effects , Iron/*metabolism , Keratinocytes/*cytology , Keratinocytes/*metabolism, Adenosine Triphosphate/metabolism ; Cell Line ; Deferoxamine/pharmacology ; Dose-Response Relationship, Radiation ; Fibroblasts/cytology ; Fibroblasts/metabolism ; Fibroblasts/radiation effects ; Hemin/pharmacology ; Humans ; Iron Chelating Agents/pharmacology ; Keratinocytes/radiation effects ; Necrosis ; Ultraviolet Rays |
| مستخلص: | The mechanism of resistance of keratinocytes to ultraviolet A (UVA) (320-400 nm)-induced oxidative damage has not yet been elucidated. Here, we examined the possible link between the intracellular level of the labile iron pool (LIP) and the susceptibility to UVA-induced cell death using a series of human skin fibroblast and keratinocyte cell lines as a model. Resistance of keratinocytes to UVA-induced cell death was confirmed by flow cytometry and in fibroblasts necrosis was found to be the primary mode of cell death induced by UVA. The percentage of necrosis in fibroblasts also correlated with the extent of intracellular ATP depletion, a hallmark of necrotic cell death. The evaluation of the intracellular level of LIP by calcein assay revealed that both "basal" and "UVA-induced" levels of LIP in keratinocytes were several fold lower than in fibroblasts. Accordingly the dose to give an equivalent level of necrosis was several fold lower in fibroblasts than in keratinocytes. Furthermore, the modulation of "basal" or "UVA-induced" level of LIP by either Desferal and/or hemin treatment significantly affected the extent of UVA-induced necrotic cell death and ATP depletion in all the cell lines. Cellular susceptibility to UVA-induced necrotic cell death appears to reflect the intracellular level of LIP. |
| المشرفين على المادة: | 0 (Iron Chelating Agents) 743LRP9S7N (Hemin) 8L70Q75FXE (Adenosine Triphosphate) E1UOL152H7 (Iron) J06Y7MXW4D (Deferoxamine) |
| تواريخ الأحداث: | Date Created: 20040918 Date Completed: 20041108 Latest Revision: 20131121 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1111/j.0022-202X.2004.23419.x |
| PMID: | 15373784 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 0022-202X |
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| DOI: | 10.1111/j.0022-202X.2004.23419.x |