دورية أكاديمية
In vivo import of yeast adenylate kinase into mitochondria affected by site-directed mutagenesis.
| العنوان: | In vivo import of yeast adenylate kinase into mitochondria affected by site-directed mutagenesis. |
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| المؤلفون: | Magdolen V; Institut für Genetik und Mikrobiologie, Munich, Germany., Schricker R, Strobel G, Germaier H, Bandlow W |
| المصدر: | FEBS letters [FEBS Lett] 1992 Mar 16; Vol. 299 (3), pp. 267-72. |
| نوع المنشور: | Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: John Wiley & Sons Ltd Country of Publication: England NLM ID: 0155157 Publication Model: Print Cited Medium: Print ISSN: 0014-5793 (Print) Linking ISSN: 00145793 NLM ISO Abbreviation: FEBS Lett Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Jan. 2016- : West Sussex : John Wiley & Sons Ltd. Original Publication: Amsterdam, North-Holland on behalf of the Federation of European Biochemical Societies. |
| مواضيع طبية MeSH: | Adenylate Kinase/*metabolism , Mitochondria/*metabolism , Saccharomyces cerevisiae/*metabolism, Adenylate Kinase/genetics ; Amino Acid Sequence ; Biological Transport, Active ; DNA Mutational Analysis ; Isoenzymes ; Molecular Sequence Data ; Mutagenesis, Site-Directed ; Saccharomyces cerevisiae/genetics ; Sequence Homology, Nucleic Acid |
| مستخلص: | Site-directed mutagenesis and deletions were used to study mitochondrial import of a major yeast adenylate kinase, Aky2p. This enzyme lacks a cleavable presequence and occurs in active and apparently unprocessed form both in mitochondria and cytoplasm. Mutations were applied to regions known to be surface-exposed and to diverge between short and long isoforms. In vertebrates, short adenylate kinase isozymes occur exclusively in the cytoplasm, whereas long versions of the enzyme have mitochondrial locations. Mutations in the extra loop of the yeast (long-form) enzyme did not affect mitochondrial import of the protein, whereas variants altered in the central, N- or C-terminal parts frequently displayed increased or, in the case of a deletion of the 8 N-terminal triplets, decreased import efficiencies. Although the N-terminus is important for targeting adenylate kinase to mitochondria, other parameters like internal sequence determinants and folding velocity of the nascent protein may also play a role. |
| المشرفين على المادة: | 0 (Isoenzymes) EC 2.7.4.3 (Adenylate Kinase) |
| تواريخ الأحداث: | Date Created: 19920316 Date Completed: 19920416 Latest Revision: 20190620 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1016/0014-5793(92)80129-5 |
| PMID: | 1544504 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 0014-5793 |
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| DOI: | 10.1016/0014-5793(92)80129-5 |