دورية أكاديمية
Early down-regulation of Bcl-xL expression during megakaryocytic differentiation of thrombopoietin-induced CD34+ bone marrow cells in essential thrombocythemia.
| العنوان: | Early down-regulation of Bcl-xL expression during megakaryocytic differentiation of thrombopoietin-induced CD34+ bone marrow cells in essential thrombocythemia. |
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| المؤلفون: | Zhang L; State Key Laboratory of Experimental Hematology, National Research Center for Stem Cell Engineering & Technology, Institute of Haematology & Blood Diseases Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin, PR China., Zhao H, Sun A, Lu S, Liu B, Tang F, Feng Y, Zhao L, Yang R, Han ZC |
| المصدر: | Haematologica [Haematologica] 2004 Oct; Vol. 89 (10), pp. 1199-206. |
| نوع المنشور: | Comparative Study; Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Ferrata Storti Foundation Country of Publication: Italy NLM ID: 0417435 Publication Model: Print Cited Medium: Internet ISSN: 1592-8721 (Electronic) Linking ISSN: 03906078 NLM ISO Abbreviation: Haematologica Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 1999- : Pavia, Italy : Ferrata Storti Foundation Original Publication: Pavia [etc.] |
| مواضيع طبية MeSH: | Down-Regulation*/drug effects, Bone Marrow Cells/*drug effects , Megakaryocytes/*pathology , Thrombocythemia, Essential/*pathology , Thrombopoietin/*pharmacology , bcl-X Protein/*physiology, Adult ; Apoptosis/drug effects ; Cell Differentiation/drug effects ; Cell Line, Tumor/drug effects ; Cell Line, Tumor/pathology ; Cells, Cultured/drug effects ; Cells, Cultured/pathology ; Culture Media, Serum-Free/pharmacology ; Female ; Gene Targeting ; Humans ; K562 Cells/drug effects ; K562 Cells/pathology ; Leukemia, Myelogenous, Chronic, BCR-ABL Positive/pathology ; Male ; Middle Aged ; Polycythemia Vera/pathology ; RNA, Small Interfering/pharmacology ; Reverse Transcriptase Polymerase Chain Reaction ; Transfection ; bcl-X Protein/biosynthesis ; bcl-X Protein/genetics |
| مستخلص: | Background and Objectives: Essential thrombocythemia (ET) is a chronic myeloproliferative disorder with abnormal megakaryocyte/platelet production. Recent studies have found that Bcl-xL, as a member of the bcl-2 family of proteins that inhibit apoptosis, is essential in megakaryocytic differentiation. In this study the expression of Bcl-xL was evaluated during megakaryocytic differentiation in ET patients. Design and Methods: To study the role of Bcl-xL in megakaryocyte differentiation, we evaluated the effect of small interfering RNA (siRNA) on the expression of Bcl-xL. CD34+ cells from patients with ET, chronic myeloid leukemia (CML), polycythemia vera (PV) and normal individuals were cultured in serum-free medium supplemented with thrombopoietin (TPO). Immunocytochemical staining and flow cytometric analysis were used to evaluate the Bcl-xL expression during megakaryocytic differentiation of CD34+ cells. Results: When exposured to si-Bcl-xL, the percentage of K562 cells induced into megakaryocytes in 72 hours was lower than the corresponding percentage of control cells. CD41a+ cells from the three groups of patients and the control group were cultured. At day 10, the percentage of Bcl-xL- cells in CD41a+ cells from ET patients was 61.0+/-28.1%, which was significantly higher than that from patients with CML (2.5+/-20.9%), PV (33.6+/-10.0%) or control subjects (15.1+/-13.0%).] Interpretation and Conclusions: These results demonstrate that Bcl-xL is down-regulated early during in vitro differentiation of megakaryocytes from ET patients; this might reflect an early entry of megakaryocytes into a degenerating mature stage. |
| المشرفين على المادة: | 0 (BCL2L1 protein, human) 0 (Culture Media, Serum-Free) 0 (RNA, Small Interfering) 0 (bcl-X Protein) 9014-42-0 (Thrombopoietin) |
| تواريخ الأحداث: | Date Created: 20041013 Date Completed: 20060616 Latest Revision: 20081121 |
| رمز التحديث: | 20221213 |
| PMID: | 15477204 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1592-8721 |
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