دورية أكاديمية
1274 full-open reading frames of transcripts expressed in the developing mouse nervous system.
| العنوان: | 1274 full-open reading frames of transcripts expressed in the developing mouse nervous system. |
|---|---|
| المؤلفون: | Bonaldo MF; Department of Pediatrics, The University of Iowa, Iowa City, Iowa 52242, USA., Bair TB, Scheetz TE, Snir E, Akabogu I, Bair JL, Berger B, Crouch K, Davis A, Eyestone ME, Keppel C, Kucaba TA, Lebeck M, Lin JL, de Melo AI, Rehmann J, Reiter RS, Schaefer K, Smith C, Tack D, Trout K, Sheffield VC, Lin JJ, Casavant TL, Soares MB |
| المصدر: | Genome research [Genome Res] 2004 Oct; Vol. 14 (10B), pp. 2053-63. |
| نوع المنشور: | Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
| اللغة: | English |
| بيانات الدورية: | Publisher: Cold Spring Harbor Laboratory Press Country of Publication: United States NLM ID: 9518021 Publication Model: Print Cited Medium: Print ISSN: 1088-9051 (Print) Linking ISSN: 10889051 NLM ISO Abbreviation: Genome Res Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Cold Spring Harbor, N.Y. : Cold Spring Harbor Laboratory Press, c1995- |
| مواضيع طبية MeSH: | Genome*, Brain/*metabolism , Eye/*metabolism , Gene Expression Regulation, Developmental/*physiology , Nerve Tissue Proteins/*genetics , Open Reading Frames/*genetics , RNA, Messenger/*genetics, Animals ; Brain/anatomy & histology ; Brain/embryology ; DNA, Complementary ; Eye/anatomy & histology ; Eye/embryology ; Female ; Gene Expression Profiling ; Gene Library ; Mice ; Mice, Inbred C57BL ; Nerve Tissue Proteins/classification ; Pregnancy ; RNA, Messenger/metabolism |
| مستخلص: | As part of the trans-National Institutes of Health (NIH) Mouse Brain Molecular Anatomy Project (BMAP), and in close coordination with the NIH Mammalian Gene Collection Program (MGC), we initiated a large-scale project to clone, identify, and sequence the complete open reading frame (ORF) of transcripts expressed in the developing mouse nervous system. Here we report the analysis of the ORF sequence of 1274 cDNAs, obtained from 47 full-length-enriched cDNA libraries, constructed by using a novel approach, herein described. cDNA libraries were derived from size-fractionated cytoplasmic mRNA isolated from brain and eye tissues obtained at several embryonic stages and postnatal days. Altogether, including the full-ORF MGC sequences derived from these libraries by the MGC sequencing team, NIH_BMAP full-ORF sequences correspond to approximately 20% of all transcripts currently represented in mouse MGC. We show that NIH_BMAP clones comprise 68% of mouse MGC cDNAs > or =5 kb, and 54% of those > or =4 kb, as of March 15, 2004. Importantly, we identified transcripts, among the 1274 full-ORF sequences, that are exclusively or predominantly expressed in brain and eye tissues, many of which encode yet uncharacterized proteins. |
| References: | Nucleic Acids Res. 1999 Dec 15;27(24):e37. (PMID: 10572189) Methods Enzymol. 1999;303:19-44. (PMID: 10349636) Genome Res. 2000 Oct;10(10):1617-30. (PMID: 11042159) Mol Genet Metab. 2001 Apr;72(4):343-50. (PMID: 11286509) Biotechniques. 2001 Jun;30(6):1250-4. (PMID: 11414214) Methods Mol Biol. 2001;175:143-53. (PMID: 11462832) Genome Res. 2001 Sep;11(9):1553-8. (PMID: 11544199) Genomics. 2001 Sep;77(1-2):79-90. (PMID: 11543636) Nat Genet. 2002 Mar;30(3):335-41. (PMID: 11810107) Genome Res. 2002 Apr;12(4):656-64. (PMID: 11932250) Biotechniques. 2002 May;32(5):984-5. (PMID: 12019793) FEBS Lett. 2002 May 22;519(1-3):71-6. (PMID: 12023020) Gene. 2002 Feb 20;285(1-2):39-47. (PMID: 12039030) Bioinformatics. 2002 Sep;18(9):1162-6. (PMID: 12217907) Nature. 2002 Dec 5;420(6915):563-73. (PMID: 12466851) Proc Natl Acad Sci U S A. 2002 Dec 24;99(26):16899-903. (PMID: 12477932) Nucleic Acids Res. 2003 Jan 1;31(1):51-4. (PMID: 12519945) Methods Mol Biol. 2003;221:73-91. (PMID: 12703735) Genome Res. 2003 Jun;13(6B):1273-89. (PMID: 12819125) Biotechniques. 2003 Sep;35(3):510-6, 518. (PMID: 14513556) Nat Genet. 2004 Jan;36(1):40-5. (PMID: 14702039) Methods Enzymol. 1980;65(1):718-49. (PMID: 6154876) Proc Natl Acad Sci U S A. 1991 Sep 1;88(17):7557-61. (PMID: 1652753) Gene. 1994 Jan 28;138(1-2):171-4. (PMID: 8125298) FEBS Lett. 1994 Oct 10;353(1):37-42. (PMID: 7926018) Brain Res Mol Brain Res. 1995 Feb;28(2):201-10. (PMID: 7723619) Cell. 1995 Jun 2;81(5):769-80. (PMID: 7539720) Neuron. 1995 Jul;15(1):63-78. (PMID: 7619531) Genome Res. 1996 Sep;6(9):791-806. (PMID: 8889548) Hum Genet. 1997 Sep;100(3-4):398-400. (PMID: 9272162) Gene. 1997 Oct 24;200(1-2):149-56. (PMID: 9373149) Genome Res. 1998 Mar;8(3):175-85. (PMID: 9521921) Genome Res. 1998 Mar;8(3):195-202. (PMID: 9521923) Brain Res Dev Brain Res. 1998 Aug 8;109(2):245-53. (PMID: 9729410) Genomics. 1998 Oct 1;53(1):81-9. (PMID: 9787079) J Neurosci. 2000 Sep 1;20(17):6333-9. (PMID: 10964938) |
| معلومات مُعتمدة: | F32 HG002881 United States HG NHGRI NIH HHS; N01MH12006 United States MH NIMH NIH HHS; 1F32HG002881-01A1 United States HG NHGRI NIH HHS |
| المشرفين على المادة: | 0 (DNA, Complementary) 0 (Nerve Tissue Proteins) 0 (RNA, Messenger) |
| تواريخ الأحداث: | Date Created: 20041019 Date Completed: 20041116 Latest Revision: 20181113 |
| رمز التحديث: | 20240628 |
| مُعرف محوري في PubMed: | PMC528920 |
| DOI: | 10.1101/gr.2601304 |
| PMID: | 15489326 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1088-9051 |
|---|---|
| DOI: | 10.1101/gr.2601304 |