دورية أكاديمية
Simian parvovirus infection: a potential zoonosis.
| العنوان: | Simian parvovirus infection: a potential zoonosis. |
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| المؤلفون: | Brown KE; Hematology Branch, National Heart, Lung, and Blood Institute, Building 10, Room CRC-3-5130, National Institutes of Health, 9000 Rockville Pike, Bethesda, MD 20892-1202, USA. brownk@nhlbi.nih.gov, Liu Z, Gallinella G, Wong S, Mills IP, O'Sullivan MG |
| المصدر: | The Journal of infectious diseases [J Infect Dis] 2004 Dec 01; Vol. 190 (11), pp. 1900-7. Date of Electronic Publication: 2004 Nov 03. |
| نوع المنشور: | Comparative Study; Journal Article; Research Support, U.S. Gov't, P.H.S. |
| اللغة: | English |
| بيانات الدورية: | Publisher: Oxford University Press Country of Publication: United States NLM ID: 0413675 Publication Model: Print-Electronic Cited Medium: Print ISSN: 0022-1899 (Print) Linking ISSN: 00221899 NLM ISO Abbreviation: J Infect Dis Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Jan. 2011- : Oxford : Oxford University Press Original Publication: 1904-2010 : Chicago, IL : University of Chicago Press |
| مواضيع طبية MeSH: | Antibodies, Viral/*blood , Bone Marrow Cells/*virology , Erythrovirus/*growth & development , Erythrovirus/*isolation & purification , Occupational Diseases/*blood , Parvoviridae Infections/*blood, Animals ; Blotting, Western ; Bone Marrow Cells/metabolism ; Capsid Proteins/biosynthesis ; Capsid Proteins/genetics ; Cells, Cultured ; Cohort Studies ; Erythrovirus/genetics ; Humans ; Macaca ; Occupational Diseases/epidemiology ; Parvoviridae Infections/epidemiology ; Parvovirus B19, Human/genetics ; Parvovirus B19, Human/metabolism ; Risk Factors ; Seroepidemiologic Studies ; Species Specificity ; Zoonoses |
| مستخلص: | Introduction: Simian parvovirus (SPV) causes severe anemia in immunocompromised macaques. The closely related erythrovirus, parvovirus B19, causes anemia in susceptible humans and can be grown in human bone marrow mononuclear cells in vitro. We hypothesized that SPV may infect humans and replicate in human bone marrow mononuclear cells. Methods: Serum samples from handlers of an SPV-seropositive macaque colony were tested by Western blot for evidence of antibodies to SPV. SPV capsid protein was expressed in insect cells, and SPV was cultured in human and macaque bone marrow mononuclear cells. Results: Fifty-one percent of exposed handlers (n=65) were found to be SPV seropositive, compared with 35% of nonexposed individuals (n=124). In 17% of exposed handlers, compared with 6% of nonexposed individuals, antibodies were directed to SPV but not to B19. SPV capsid proteins, like those of B19, self-assembled to form parvovirus-like particles, and these capsids, like B19 capsids, bound to globoside, suggesting that globoside is also the receptor for SPV. We demonstrated that SPV could replicate in vitro in both human and macaque bone marrow mononuclear cells and that it was cytotoxic to erythroid progenitor cells. Conclusions: Our data suggest that SPV may infect human bone marrow mononuclear cells in vitro and in vivo and should be considered a potential zoonosis. |
| المشرفين على المادة: | 0 (Antibodies, Viral) 0 (Capsid Proteins) |
| تواريخ الأحداث: | Date Created: 20041106 Date Completed: 20041230 Latest Revision: 20061115 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1086/425420 |
| PMID: | 15529252 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 0022-1899 |
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| DOI: | 10.1086/425420 |