دورية أكاديمية
Development of ecdysone-regulated lentiviral vectors.
| العنوان: | Development of ecdysone-regulated lentiviral vectors. |
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| المؤلفون: | Galimi F; Laboratory of Genetics, The Salk Institute for Biological Studies, 10110 North Torrey Pines Road, La Jolla, CA 92037, USA., Saez E, Gall J, Hoong N, Cho G, Evans RM, Verma IM |
| المصدر: | Molecular therapy : the journal of the American Society of Gene Therapy [Mol Ther] 2005 Jan; Vol. 11 (1), pp. 142-8. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
| اللغة: | English |
| بيانات الدورية: | Publisher: Cell Press Country of Publication: United States NLM ID: 100890581 Publication Model: Print Cited Medium: Print ISSN: 1525-0016 (Print) Linking ISSN: 15250016 NLM ISO Abbreviation: Mol Ther Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2017- : Cambridge, MA : Cell Press Original Publication: San Diego, CA : Academic Press, 2000- |
| مواضيع طبية MeSH: | Ecdysone/*pharmacology , Gene Expression Regulation/*genetics , Genetic Vectors/*genetics , Lentivirus/*genetics, Animals ; Bone Marrow Transplantation ; Cell Line ; Ecdysone/metabolism ; Hematopoietic Stem Cells/drug effects ; Hematopoietic Stem Cells/metabolism ; Humans ; Mice ; Mice, Inbred C57BL ; Promoter Regions, Genetic/genetics ; Receptors, Steroid/genetics ; Receptors, Steroid/metabolism ; Transduction, Genetic |
| مستخلص: | We have engineered a lentivirus-based gene transfer system to achieve ecdysone-regulated transgene expression. The method combines the wide tropism of lentiviral vectors and the possibility of gene regulation by a small molecule with an excellent pharmacological profile. Using the hematopoietic tissue as a model, we transduced mouse progenitors with an ecdysone-regulated GFP expression cassette. The ecdysone gene switch efficiently turned GFP on and off in transplanted animals, showing low basal activity. This system allows the delivery of inducible transcriptional units in vitro and ex vivo and may be a useful tool for gene transfer purposes. Moreover, our work provides hints on the design of lentiviral vectors containing multiple expression cassettes with multiple promoters. |
| المشرفين على المادة: | 0 (Receptors, Steroid) 0 (ecdysone receptor) 3604-87-3 (Ecdysone) |
| تواريخ الأحداث: | Date Created: 20041209 Date Completed: 20050504 Latest Revision: 20211103 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1016/j.ymthe.2004.08.021 |
| PMID: | 15585415 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1525-0016 |
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| DOI: | 10.1016/j.ymthe.2004.08.021 |