دورية أكاديمية
Immunological identification of Fasciola hepatica antigens containing major human T-cell and B-cell epitopes.
| العنوان: | Immunological identification of Fasciola hepatica antigens containing major human T-cell and B-cell epitopes. |
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| المؤلفون: | Mohamed MM; Department of Zoology, Faculty of Science, Cairo University, Cairo, Egypt. monamoa@hotmail.com, Al-Sherbiny MM, Sharaf AA, Elmamlouk TH |
| المصدر: | Journal of the Egyptian Society of Parasitology [J Egypt Soc Parasitol] 2004 Dec; Vol. 34 (3), pp. 751-66. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: The Society] Country of Publication: Egypt NLM ID: 8102141 Publication Model: Print Cited Medium: Print ISSN: 1110-0583 (Print) Linking ISSN: 11100583 NLM ISO Abbreviation: J Egypt Soc Parasitol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: [Cairo : The Society] |
| مواضيع طبية MeSH: | Antigens, Helminth/*immunology , Epitopes, B-Lymphocyte/*immunology , Epitopes, T-Lymphocyte/*immunology , Fasciola hepatica/*immunology , Fascioliasis/*immunology, Animals ; Antigens, Helminth/analysis ; Fascioliasis/parasitology ; Female ; Humans ; Male |
| مستخلص: | Fasciola hepatica whole worm homogenate (Fhwwh) separated fractions were used in enzyme linked immunoelectrotransfer blot (EITB) to identify the antigen(s) which induces antibody formation in human fascioliasis. The immuno-reactive antigens recognized by the infected patients were 25-29 kDa and 12 kDa. Antigens were biochemically purified by model 491-prep cell fraction (BIO-RAD). The capability of the purified target antigens to induce humoral and cellular responses with cells and sera of infected patients was investigated using enzyme linked immunosorbent assay (ELISA) and lymphoproliferative responses techniques. The 25-29 kDa cluster of antigen(s) were found to be more efficient in inducing lymphoproliferative response than 12 KDa, thus, it was considered as the target antigens used in the generation of human monospecific polyclonal immunopurified antibody probes (IPAb). The specificity and immunoreactivity of the IPAb with Fhwwh, F. hepatica excretory secretory products (FhESP) and S. mansoni adult worm antigen (SAWA) were evaluated by using EITB. Results showed that IPAb was immunoreactive with 25-29 and 12 kDa antigens of both Fhwwh and FhESP. It was concluded that the most immunogenic F. hepatica target antigen(s) were 25-29 and12 KDa antigens and there is a cross-reactivity between 25-29 and 12 KDa antigens in both Fhwwh and FhESP. The involvement of IPAb in antibody dependent cell mediated cytotoxicity in-vitro technique was studied. Results indicated that human neturophils were more effective in adhering to the IPAb-coated flukes at early stages of development. |
| المشرفين على المادة: | 0 (Antigens, Helminth) 0 (Epitopes, B-Lymphocyte) 0 (Epitopes, T-Lymphocyte) |
| تواريخ الأحداث: | Date Created: 20041214 Date Completed: 20050224 Latest Revision: 20130502 |
| رمز التحديث: | 20240628 |
| PMID: | 15587304 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1110-0583 |
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