دورية أكاديمية
Comparison of morphology, viability, and function between blood and milk neutrophils from peak lactating goats.
| العنوان: | Comparison of morphology, viability, and function between blood and milk neutrophils from peak lactating goats. |
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| المؤلفون: | Tian SZ; Department of Animal Science, National Chung Hsing University, Taichung, Taiwan., Chang CJ, Chiang CC, Peh HC, Huang MC, Lee JW, Zhao X |
| المصدر: | Canadian journal of veterinary research = Revue canadienne de recherche veterinaire [Can J Vet Res] 2005 Jan; Vol. 69 (1), pp. 39-45. |
| نوع المنشور: | Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Canadian Veterinary Medical Association Country of Publication: Canada NLM ID: 8607793 Publication Model: Print Cited Medium: Print ISSN: 0830-9000 (Print) Linking ISSN: 08309000 NLM ISO Abbreviation: Can J Vet Res Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Ottawa, Ont. : Canadian Veterinary Medical Association, c1986- |
| مواضيع طبية MeSH: | Apoptosis/*physiology , Goats/*physiology , Milk/*cytology , Neutrophils/*cytology, Animals ; Female ; Flow Cytometry/veterinary ; Goats/blood ; Goats/immunology ; Lactation ; Microscopy, Electron, Scanning/veterinary ; Milk/immunology ; Necrosis ; Neutrophils/immunology ; Neutrophils/pathology ; Neutrophils/ultrastructure ; Reactive Oxygen Species/analysis ; Reactive Oxygen Species/metabolism |
| مستخلص: | The morphological features of blood and milk neutrophils from peak lactating goats were compared using light microscopy, scanning electron microscopy and flow cytometry in order to investigate the cytological changes of neutrophils after migration into the mammary gland. The kinetics of reactive oxygen intermediates (ROI) generation and gelatinase release of blood and milk neutrophils, with or without stimulation of phorbol 12-myristate, 13-acetate ester (PMA), were used to characterize their responses to inflammatory stimuli. Neutrophils isolated from goat milk were highly segmented and contained multi-lobed nuclei. Ultrastructurally, milk neutrophils were more ruffled on the surface compared to blood neutrophils. Approximately 30% of milk neutrophils were undergoing cell death, either necrosis or apoptosis, in contrast to 8% of blood neutrophils. The ROI production of activated milk neutrophils peaked earlier than blood neutrophils, but the duration and the intensity were much less. Neutrophils from both sources augmented the release of gelatinase in response to PMA (1 ng/mL). However, the amount of gelatinase released from milk neutrophils was lower (P < 0.05) than that of blood neutrophils. In summary, more neutrophils become apoptotic and necrotic in the mammary gland, presumably due to spontaneous aging, the process of diapedesis, and the interaction with milk components. Milk neutrophils have impaired functionalities in comparison with blood neutrophils. The information is relevant when studying mammary gland immunity and related diseases, such as mastitis. |
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| المشرفين على المادة: | 0 (Reactive Oxygen Species) |
| تواريخ الأحداث: | Date Created: 20050305 Date Completed: 20050705 Latest Revision: 20181113 |
| رمز التحديث: | 20231215 |
| مُعرف محوري في PubMed: | PMC1142168 |
| PMID: | 15745221 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 0830-9000 |
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