دورية أكاديمية
Magnifection--a new platform for expressing recombinant vaccines in plants.
| العنوان: | Magnifection--a new platform for expressing recombinant vaccines in plants. |
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| المؤلفون: | Gleba Y; Icon Genetics, Biozentrum Halle, Weinbergweg 22, D-06120 Halle (Saale), Germany. gleba@icongenetics.de, Klimyuk V, Marillonnet S |
| المصدر: | Vaccine [Vaccine] 2005 Mar 18; Vol. 23 (17-18), pp. 2042-8. |
| نوع المنشور: | Journal Article; Review |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Science Country of Publication: Netherlands NLM ID: 8406899 Publication Model: Print Cited Medium: Print ISSN: 0264-410X (Print) Linking ISSN: 0264410X NLM ISO Abbreviation: Vaccine Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Amsterdam, The Netherlands : Elsevier Science Original Publication: [Guildford, Surrey, UK] : Butterworths, [c1983- |
| مواضيع طبية MeSH: | Plants, Genetically Modified/*genetics , Plants, Genetically Modified/*immunology , Transfection/*methods , Vaccines, Synthetic/*biosynthesis , Vaccines, Synthetic/*genetics, Gene Expression ; Genetic Engineering ; Genetic Techniques ; Genetic Vectors ; Green Fluorescent Proteins/biosynthesis ; Green Fluorescent Proteins/genetics ; Plant Viruses/genetics ; Plastids/genetics ; Recombinant Proteins/biosynthesis ; Recombinant Proteins/genetics ; Recombinant Proteins/immunology ; Safety ; Vaccines, Synthetic/isolation & purification |
| مستخلص: | Today, plant biotechnology relies on two processes for delivery and expression of heterologous genes in plants: stable genetic transformation and transient infection with viral vectors. Although much faster, the transient route until recently was limited because of virus' low infectivity and its inability to carry average-size or larger transgenes. A recently developed new generation transfection technology overcomes these limitations by relying on Agrobacterium as an infective systemic agent that delivers viral replicons. This improved process is being used to simultaneously start transient gene amplification and high-level expression in all mature leaves of a plant, and such a transfection can be done on an industrial scale. This eclectic technology, called 'magnifection', combines advantages of three biological systems: vector efficiency and efficient systemic DNA delivery of Agrobacterium, speed and expression level/yield of a plant RNA virus, as well as posttranslational capabilities and low production costs of a plant. The proposed process allows for industrial production that does not require genetic modification of plants, that is much faster than previous methods, and that is biologically safe. Numerous applications in the area of vaccine manufacturing are being discussed. |
| Number of References: | 17 |
| المشرفين على المادة: | 0 (Recombinant Proteins) 0 (Vaccines, Synthetic) 147336-22-9 (Green Fluorescent Proteins) |
| تواريخ الأحداث: | Date Created: 20050310 Date Completed: 20050518 Latest Revision: 20201226 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1016/j.vaccine.2005.01.006 |
| PMID: | 15755568 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 0264-410X |
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| DOI: | 10.1016/j.vaccine.2005.01.006 |