دورية أكاديمية
Removal of aqueous phenol using immobilized enzymes in a bench scale and pilot scale three-phase fluidized bed reactor.
| العنوان: | Removal of aqueous phenol using immobilized enzymes in a bench scale and pilot scale three-phase fluidized bed reactor. |
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| المؤلفون: | Ensuncho L; Department of Civil Engineering, University of Waterloo, Waterloo, ON, Canada, N2L 3G1., Alvarez-Cuenca M, Legge RL |
| المصدر: | Bioprocess and biosystems engineering [Bioprocess Biosyst Eng] 2005 May; Vol. 27 (3), pp. 185-91. Date of Electronic Publication: 2005 Mar 12. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Springer-Verlag Country of Publication: Germany NLM ID: 101088505 Publication Model: Print-Electronic Cited Medium: Print ISSN: 1615-7591 (Print) Linking ISSN: 16157591 NLM ISO Abbreviation: Bioprocess Biosyst Eng Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Berlin, Germany : Springer-Verlag, 2001- |
| مواضيع طبية MeSH: | Enzymes, Immobilized/*chemistry , Fungal Proteins/*chemistry , Monophenol Monooxygenase/*chemistry , Phenol/*chemistry, Alginates/chemistry ; Chitosan/chemistry ; Glucuronic Acid/chemistry ; Hexuronic Acids/chemistry ; Waste Disposal, Fluid/methods ; Water Purification/methods |
| مستخلص: | The main objective of this work was to investigate the removal of aqueous phenol using immobilized enzymes in both bench scale and pilot scale three-phase fluidized bed reactors. The enzyme used in this application was a fungal tyrosinase [E.C. 1.14.18.1] immobilized in a system of chitosan and alginate. The immobilization matrix consisted of a chitosan matrix cross-linked with glutaraldehyde with an aliginate-filled pore space. This support matrix showed superior mechanical properties along with retaining the unique adsorptive characteristics of the chitosan. Adsorption of the o-quinone product by the chitosan reduced tyrosinase inactivation that is normally observed for this enzyme under these conditions. This approach allowed reuse of the enzyme in repeated batch applications. For the bench scale reactor (1.2-l capacity) more than 92% of the phenol could be removed from the feed water using an immobilized enzyme volume of 18.5% and a residence time of the liquid phase of 150 min. Removal rates decreased with subsequent batch runs. For the pilot scale fluidized bed (60 l), 60% phenol removal was observed with an immobilized enzyme volume of 5% and a residence time of the liquid phase of 7 h. Removal decreased to 45% with a repeat batch run with the same immobilized enzyme. |
| المشرفين على المادة: | 0 (Alginates) 0 (Enzymes, Immobilized) 0 (Fungal Proteins) 0 (Hexuronic Acids) 339NCG44TV (Phenol) 8A5D83Q4RW (Glucuronic Acid) 9012-76-4 (Chitosan) EC 1.14.18.1 (Monophenol Monooxygenase) |
| تواريخ الأحداث: | Date Created: 20050315 Date Completed: 20050826 Latest Revision: 20181201 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1007/s00449-005-0400-x |
| PMID: | 15765216 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1615-7591 |
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| DOI: | 10.1007/s00449-005-0400-x |