دورية أكاديمية
Modulation of homing properties of primitive progenitor cells generated by ex vivo expansion.
| العنوان: | Modulation of homing properties of primitive progenitor cells generated by ex vivo expansion. |
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| المؤلفون: | Foguenne J; Department of Medicine/Hematology, University of Liège, Liège, Belgium., Huygen S, Greimers R, Beguin Y, Gothot A |
| المصدر: | Haematologica [Haematologica] 2005 Apr; Vol. 90 (4), pp. 445-51. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Ferrata Storti Foundation Country of Publication: Italy NLM ID: 0417435 Publication Model: Print Cited Medium: Internet ISSN: 1592-8721 (Electronic) Linking ISSN: 03906078 NLM ISO Abbreviation: Haematologica Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 1999- : Pavia, Italy : Ferrata Storti Foundation Original Publication: Pavia [etc.] |
| مواضيع طبية MeSH: | Cell Adhesion Molecules/*metabolism , Hematopoietic Stem Cells/*cytology, AC133 Antigen ; Animals ; Antigens, CD/blood ; Bone Marrow ; Cell Division ; Cell Movement ; Cells, Cultured ; Down-Regulation ; Fibronectins/metabolism ; Glycoproteins/blood ; Hematopoietic Stem Cell Transplantation ; Integrin alpha4beta1/physiology ; Mice ; Mice, Inbred NOD ; Mice, SCID ; Mitosis/physiology ; Peptides/blood ; Receptors, Lymphocyte Homing ; Vascular Cell Adhesion Molecule-1/metabolism |
| مستخلص: | Background and Objectives: The maintenance of adequate interactions with the bone marrow (BM) microenvironment is critical to ensure efficient homing of ex vivo-expanded hematopoietic cells. This study was intended to assess adhesion and migration properties of long-term culture-initiating cells (LTC-IC) harvested after self-renewal division in ex vivo culture and to determine their susceptibility to growth-inhibitory signals mediated by adhesion to BM stromal ligands. Design and Methods: We used cell tracking to isolate primitive LTC-IC that had accomplished 1 or 2 divisions ex vivo. Adhesion, migration and growth inhibition of divided LTC-IC were determined in the presence of purified BM ligands, and compared to the properties of uncultured LTC-IC. Results: As compared to undivided LTC-IC, adhesion and migration mediated by very late antigen (VLA)-4 integrin across both vascular cell adhesion molecule-1 (VCAM-1) and fibronectin (Fn) were downregulated in post-mitotic LTC-IC. Conversely, binding and motility via VLA-5 across Fn were stimulated. No changes occurred in LTC-IC interactions with intercellular adhesion molecule-1 (ICAM-1) or with E- or P-selectin. Proliferation of uncultured LTC-IC was inhibited by VLA-4-mediated binding to VCAM-1 and the CS-1 domain of Fn, as well as binding to P-selectin. Growth of ex vivo-generated LTC-IC became unresponsive to these 3 ligands but was suppressed through VLA-5 engagement by the cell binding domain of Fn. Interpretation and Conclusions: The generation of LTC-IC in expansion culture is associated with functional alterations of adhesion receptors, modulating not only binding and migration in the BM but also responsiveness to adhesion-mediated growth inhibitory signals. Such changes may limit homing and engraftment of expanded primitive stem/progenitor cells. |
| التعليقات: | Comment in: Haematologica. 2005 Apr;90(4):433. (PMID: 15820925) |
| المشرفين على المادة: | 0 (AC133 Antigen) 0 (Antigens, CD) 0 (Cell Adhesion Molecules) 0 (Fibronectins) 0 (Glycoproteins) 0 (Integrin alpha4beta1) 0 (Peptides) 0 (Receptors, Lymphocyte Homing) 0 (Vascular Cell Adhesion Molecule-1) |
| تواريخ الأحداث: | Date Created: 20050412 Date Completed: 20060525 Latest Revision: 20161124 |
| رمز التحديث: | 20240628 |
| PMID: | 15820938 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1592-8721 |
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