دورية أكاديمية
Frequency of beryllium-specific, TH1-type cytokine-expressing CD4+ T cells in patients with beryllium-induced disease.
| العنوان: | Frequency of beryllium-specific, TH1-type cytokine-expressing CD4+ T cells in patients with beryllium-induced disease. |
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| المؤلفون: | Pott GB; Department of Medicine, University of Colorado Health Sciences Center, Denver, CO 80262, USA., Palmer BE, Sullivan AK, Silviera L, Maier LA, Newman LS, Kotzin BL, Fontenot AP |
| المصدر: | The Journal of allergy and clinical immunology [J Allergy Clin Immunol] 2005 May; Vol. 115 (5), pp. 1036-42. |
| نوع المنشور: | Comparative Study; Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, P.H.S. |
| اللغة: | English |
| بيانات الدورية: | Publisher: Mosby Country of Publication: United States NLM ID: 1275002 Publication Model: Print Cited Medium: Print ISSN: 0091-6749 (Print) Linking ISSN: 00916749 NLM ISO Abbreviation: J Allergy Clin Immunol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: St Louis, Mosby. |
| مواضيع طبية MeSH: | Immunization*, Allergens/*adverse effects , Beryllium/*adverse effects , CD4-Positive T-Lymphocytes/*immunology , Hypersensitivity/*immunology, Adult ; Aged ; Aged, 80 and over ; Bronchoalveolar Lavage Fluid/immunology ; Cells, Cultured ; Disease Progression ; Female ; Humans ; Hypersensitivity/diagnosis ; Interferon-gamma/biosynthesis ; Interleukin-2/biosynthesis ; Lymphocyte Count ; Male ; Middle Aged ; Species Specificity |
| مستخلص: | Background: Beryllium sensitization is caused by exposure to beryllium in the workplace. A subset of beryllium-sensitized (BeS) subjects progress to chronic beryllium disease (CBD), a disorder characterized by a CD4+ T-cell alveolitis and granulomatous inflammation. Whether biomarkers are present in blood that would allow separation of CBD from beryllium sensitization without invasive pulmonary procedures is unknown. Objective: The aim of this study was to determine whether the quantity of beryllium-specific T cells in blood of patients with CBD differs from that found in BeS subjects. Methods: Beryllium-induced T-cell proliferation and T H 1-type cytokine secretion were determined in blood cells from 33 patients with CBD and 18 BeS subjects. Results: We demonstrate here that patients with CBD have a significantly elevated number of IFN-gamma-producing and IL-2-producing beryllium-specific CD4+ T cells in blood compared with both BeS and normal control subjects. In contrast, no difference in beryllium-induced proliferation of blood T cells was seen between BeS patients and patients with CBD. Compared with the blood beryllium lymphocyte proliferation test, which detected beryllium-induced proliferation in 65% of BeS patients and patients with CBD, ELISPOT analysis detected IFN-gamma secretion in 80% of these subjects. Higher numbers of beryllium-specific cells in blood were also associated with the extent of alveolar inflammation, as measured by both bronchoalveolar lavage white blood cell and lymphocyte counts. Conclusion: The frequency of beryllium-specific T cells in the blood of beryllium-exposed subjects may be a useful biomarker that helps discriminate between beryllium sensitization and progression to CBD. |
| معلومات مُعتمدة: | R01 ES06358 United States ES NIEHS NIH HHS; P01 ES011810 United States ES NIEHS NIH HHS; R01 HL062410 United States HL NHLBI NIH HHS; M01-RR-0051 United States RR NCRR NIH HHS; R01 HL62410 United States HL NHLBI NIH HHS |
| المشرفين على المادة: | 0 (Allergens) 0 (Interleukin-2) 82115-62-6 (Interferon-gamma) OW5102UV6N (Beryllium) |
| تواريخ الأحداث: | Date Created: 20050504 Date Completed: 20050622 Latest Revision: 20161208 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1016/j.jaci.2005.01.019 |
| PMID: | 15867863 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 0091-6749 |
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| DOI: | 10.1016/j.jaci.2005.01.019 |