دورية أكاديمية
4-Hydroxy-oxyphenbutazone is a potent inhibitor of cytokine production.
| العنوان: | 4-Hydroxy-oxyphenbutazone is a potent inhibitor of cytokine production. |
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| المؤلفون: | Ten Brinke A; Department of Immunopathology, Sanquin Research at CLB, PO.BOX 9190, 1006 AD Amsterdam, The Netherlands. a.tenbrinke@sanquin.nl, Dekkers DW, Notten SM, Karsten ML, de Groot ER, Aarden LA |
| المصدر: | European cytokine network [Eur Cytokine Netw] 2005 Jun; Vol. 16 (2), pp. 144-51. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: John Libbey Eurotext Ltd Country of Publication: France NLM ID: 9100879 Publication Model: Print Cited Medium: Print ISSN: 1148-5493 (Print) Linking ISSN: 11485493 NLM ISO Abbreviation: Eur Cytokine Netw Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Montrouge, France : John Libbey Eurotext Ltd. |
| مواضيع طبية MeSH: | Granulocyte-Macrophage Colony-Stimulating Factor/*antagonists & inhibitors , Interleukin-6/*antagonists & inhibitors , Oxyphenbutazone/*analogs & derivatives, Cell Survival/drug effects ; Cells, Cultured ; Erythrocytes/drug effects ; Erythrocytes/metabolism ; Granulocyte-Macrophage Colony-Stimulating Factor/biosynthesis ; Humans ; In Vitro Techniques ; Interleukin-6/biosynthesis ; Monocytes/drug effects ; Monocytes/metabolism ; Oxyphenbutazone/pharmacology ; T-Lymphocytes/drug effects ; T-Lymphocytes/metabolism |
| مستخلص: | 4-Hydroxy-oxyphenbutazone (4OH-OPB), is currently in phase II trials for its immunosuppressive effect in patients with rheumatoid arthritis. 4OH-OPB and other compounds related to phenylbutazone were tested for their effect on in vitro cytokine production by monocytes and lymphocytes present in peripheral mononuclear cells (PBMC) or whole blood (WB) cultures, and compared against phenylbutazone and oxyphenbutazone, two known anti-inflammatory drugs. In PBMC cultures, 4OH-OPB was by far the most potent inhibitor, and both monokines and Th1 and Th2 lymphokines were efficiently inhibited at low concentrations. In WB cultures, 4OH-OPB was less effective than in PBMC cultures, but was still the best inhibitor of lymphokine production and, furthermore, was the only inhibitor of monokine production. The increase in 4OH-OPB concentration needed to induce the same inhibition of cytokine production in WB as in PBMC culture could be mimicked by the addition of erythrocytes to the PBMC cultures. Experiments with radioactively-labeled 4OH-OPB suggest that 4OH-OPB is taken up very rapidly into erythrocytes and is secreted by the erythrocytes with much slower kinetics via a multidrug-resistance-associated protein. The secreted compound is most likely structurally different from 4OH-OPB, as in PBMC and WB cultures, the inhibition of cytokine production seems to be caused by a different mechanism. In PBMC cultures, the inhibition of cytokine production is accompanied by a loss of cell viability, while this is not the case when 4OH-OPB inhibits cytokine production in WB. Our data suggest that 4OH-OPB may be useful as an immunosuppressive drug for patients with inflammatory diseases. |
| المشرفين على المادة: | 0 (4-hydroxy-oxyphenbutazone) 0 (Interleukin-6) 83869-56-1 (Granulocyte-Macrophage Colony-Stimulating Factor) H806S4B3NS (Oxyphenbutazone) |
| تواريخ الأحداث: | Date Created: 20050609 Date Completed: 20051123 Latest Revision: 20141120 |
| رمز التحديث: | 20221213 |
| PMID: | 15941686 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1148-5493 |
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