دورية أكاديمية
[The characteristics of nuclear/cytoplasmic genomes of asymmetric somatic hybrid of Daucus carota var. sativa and Swertia mussotii].
العنوان: | [The characteristics of nuclear/cytoplasmic genomes of asymmetric somatic hybrid of Daucus carota var. sativa and Swertia mussotii]. |
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المؤلفون: | Li ZD; School of Life Sciences, Shandong Univeristy, Jinan 250100, China., Shao F, Cai YF, Xiang FN, Xia GM |
المصدر: | Zhi wu sheng li yu fen zi sheng wu xue xue bao = Journal of plant physiology and molecular biology [Zhi Wu Sheng Li Yu Fen Zi Sheng Wu Xue Xue Bao] 2005 Jun; Vol. 31 (3), pp. 254-60. |
نوع المنشور: | English Abstract; Journal Article; Research Support, Non-U.S. Gov't |
اللغة: | Chinese |
بيانات الدورية: | Publisher: Ke xue chu ban she Country of Publication: China NLM ID: 101156321 Publication Model: Print Cited Medium: Print ISSN: 1671-3877 (Print) Linking ISSN: 16713877 NLM ISO Abbreviation: Zhi Wu Sheng Li Yu Fen Zi Sheng Wu Xue Xue Bao Subsets: MEDLINE |
أسماء مطبوعة: | Original Publication: Beijing : Ke xue chu ban she, 2002- |
مواضيع طبية MeSH: | Daucus carota/*genetics , Genome, Plant/*genetics , Swertia/*genetics, Chimera/genetics ; DNA, Ribosomal Spacer/chemistry ; DNA, Ribosomal Spacer/genetics ; Daucus carota/radiation effects ; RNA, Ribosomal, 5S/genetics ; Random Amplified Polymorphic DNA Technique ; Sequence Analysis, DNA ; Swertia/radiation effects |
مستخلص: | Protoplasts of Swertia mussotii irradiated by ultra-violet light (UV) 260 microW/cm(2) were fused with protoplasts of Daucus carota var. sativa using the PEG method. Analysis of 5S rDNA spacer sequences and random amplified polymorphic DNA (RAPD) showed the presence and combination of nuclear DNA from both parents. All hybrids mainly contained genomes of D. carota (receptor) RAPD bands, and a few donor RAPD bands of S. mussotii. There is no obvious relationship between the nuclear DNA composition was not affected by UV-dosage. SSR analysis of chloroplast DNA of the hybrid clones further revealed that cpDNAs of the parents were integrated and recombined in the hybrids randomly. |
المشرفين على المادة: | 0 (DNA, Ribosomal Spacer) 0 (RNA, Ribosomal, 5S) |
تواريخ الأحداث: | Date Created: 20050618 Date Completed: 20090319 Latest Revision: 20050617 |
رمز التحديث: | 20221213 |
PMID: | 15961899 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1671-3877 |
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