دورية أكاديمية
Ebselen protects glutamate uptake inhibition caused by methyl mercury but does not by Hg2+.
| العنوان: | Ebselen protects glutamate uptake inhibition caused by methyl mercury but does not by Hg2+. |
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| المؤلفون: | Moretto MB; Departamento de Análises Clínicas e Toxicológicas, Centro de Ciências da Saúde, Universidade Federal de Santa Maria, RS, Brazil., Funchal C, Santos AQ, Gottfried C, Boff B, Zeni G, Pureur RP, Souza DO, Wofchuk S, Rocha JB |
| المصدر: | Toxicology [Toxicology] 2005 Oct 15; Vol. 214 (1-2), pp. 57-66. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: Ireland NLM ID: 0361055 Publication Model: Print Cited Medium: Print ISSN: 0300-483X (Print) Linking ISSN: 0300483X NLM ISO Abbreviation: Toxicology Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Limerick : Elsevier Original Publication: Amsterdam. Elsevier/North-Holland. |
| مواضيع طبية MeSH: | Azoles/*pharmacology , Cerebral Cortex/*drug effects , Glutamic Acid/*metabolism , Mercuric Chloride/*toxicity , Methylmercury Compounds/*toxicity , Neuroprotective Agents/*pharmacology , Organoselenium Compounds/*pharmacology, Animals ; Benzene Derivatives/pharmacology ; Cell Survival/drug effects ; Cerebral Cortex/growth & development ; Cerebral Cortex/metabolism ; In Vitro Techniques ; Isoindoles ; Rats ; Rats, Wistar |
| مستخلص: | Alterations of the neurotransmitter release systems in CNS have been reported in a variety of neuropathological processes associated with heavy metal toxicity. Neurotoxic effects of mercurials were investigated in vitro in cerebral cortex slices from young rats. The present study indicates that: (i) the environmental contaminants methylmercury (MeHg) and mercuric chloride (Hg2+) (50 microM) inhibited the glutamate net uptake from the cerebral cortex of 17-day-old rats; (ii) ebselen (10 microM) reverted the MeHg-induced inhibition of glutamate net uptake but did not protect the inhibition caused by Hg2+. At same time, we investigated another diorganochalcogenide, diphenyl diselenide (PhSe)2 and it was observed that this compound did not revert the action of MeHg or Hg2+; (iii) in addition, we observed that exposure of slices to 50 microM MeHg and Hg2+ for 30 min followed by Trypan blue exclusion assay resulted in 58.5 and 67.5% of staining cells, respectively, indicating a decrease in cell viability. Ebselen protected slices from the deleterious effects of MeHg, but not of Hg2+ on cell viability. Conversely, ebselen did not modify the reduction of MTT caused by MeHg and Hg2+; (iv) the protective effect of ebselen on MeHg-induced inhibition of glutamate net uptake seems to be related to its ability in maintaining cell viability. |
| المشرفين على المادة: | 0 (Azoles) 0 (Benzene Derivatives) 0 (Isoindoles) 0 (Methylmercury Compounds) 0 (Neuroprotective Agents) 0 (Organoselenium Compounds) 1666-13-3 (diphenyldiselenide) 3KX376GY7L (Glutamic Acid) 40X2P7DPGH (ebselen) 53GH7MZT1R (Mercuric Chloride) |
| تواريخ الأحداث: | Date Created: 20050714 Date Completed: 20051128 Latest Revision: 20211203 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1016/j.tox.2005.05.022 |
| PMID: | 16011868 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 0300-483X |
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| DOI: | 10.1016/j.tox.2005.05.022 |