دورية أكاديمية
Production of human monoclonal antibody in eggs of chimeric chickens.
| العنوان: | Production of human monoclonal antibody in eggs of chimeric chickens. |
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| المؤلفون: | Zhu L; Origen Therapeutics, 1450 Rollins Road, Burlingame, California 94010, USA., van de Lavoir MC, Albanese J, Beenhouwer DO, Cardarelli PM, Cuison S, Deng DF, Deshpande S, Diamond JH, Green L, Halk EL, Heyer BS, Kay RM, Kerchner A, Leighton PA, Mather CM, Morrison SL, Nikolov ZL, Passmore DB, Pradas-Monne A, Preston BT, Rangan VS, Shi M, Srinivasan M, White SG, Winters-Digiacinto P, Wong S, Zhou W, Etches RJ |
| المصدر: | Nature biotechnology [Nat Biotechnol] 2005 Sep; Vol. 23 (9), pp. 1159-69. Date of Electronic Publication: 2005 Aug 28. |
| نوع المنشور: | Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, P.H.S. |
| اللغة: | English |
| بيانات الدورية: | Publisher: Nature America Publishing Country of Publication: United States NLM ID: 9604648 Publication Model: Print-Electronic Cited Medium: Print ISSN: 1087-0156 (Print) Linking ISSN: 10870156 NLM ISO Abbreviation: Nat Biotechnol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: New York Ny : Nature America Publishing Original Publication: New York, NY : Nature Pub. Co., [1996- |
| مواضيع طبية MeSH: | Antibodies, Monoclonal/*chemistry, Animals ; Blotting, Southern ; Blotting, Western ; CHO Cells ; Calorimetry, Differential Scanning ; Carbohydrates/chemistry ; Chickens ; Cricetinae ; DNA/metabolism ; Egg White ; Embryo, Mammalian/cytology ; Embryo, Nonmammalian ; Enzyme-Linked Immunosorbent Assay ; Female ; Genetic Vectors ; Genome ; Glycosylation ; Humans ; Immunoglobulin G ; Immunohistochemistry ; Isoelectric Focusing ; Mice ; Mice, Inbred BALB C ; Models, Genetic ; Monosaccharides/chemistry ; Oligosaccharides/chemistry ; Ovalbumin/genetics ; Ovalbumin/metabolism ; Polymerase Chain Reaction ; Recombinant Fusion Proteins/chemistry ; Reverse Transcriptase Polymerase Chain Reaction ; Spectrometry, Mass, Electrospray Ionization ; Stem Cells/cytology |
| مستخلص: | The tubular gland of the chicken oviduct is an attractive system for protein expression as large quantities of proteins are deposited in the egg, the production of eggs is easily scalable and good manufacturing practices for therapeutics from eggs have been established. Here we examined the ability of upstream and downstream DNA sequences of ovalbumin, a protein produced exclusively in very high quantities in chicken egg white, to drive tissue-specific expression of human mAb in chicken eggs. To accommodate these large regulatory regions, we established and transfected lines of chicken embryonic stem (cES) cells and formed chimeras that express mAb from cES cell-derived tubular gland cells. Eggs from high-grade chimeras contained up to 3 mg of mAb that possesses enhanced antibody-dependent cellular cytotoxicity (ADCC), nonantigenic glycosylation, acceptable half-life, excellent antigen recognition and good rates of internalization. |
| معلومات مُعتمدة: | AI29470 United States AI NIAID NIH HHS; AI39187 United States AI NIAID NIH HHS; AI51415 United States AI NIAID NIH HHS; GM064261 United States GM NIGMS NIH HHS |
| المشرفين على المادة: | 0 (Antibodies, Monoclonal) 0 (Carbohydrates) 0 (Immunoglobulin G) 0 (Monosaccharides) 0 (Oligosaccharides) 0 (Recombinant Fusion Proteins) 9006-59-1 (Ovalbumin) 9007-49-2 (DNA) |
| تواريخ الأحداث: | Date Created: 20050830 Date Completed: 20051221 Latest Revision: 20071115 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1038/nbt1132 |
| PMID: | 16127450 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1087-0156 |
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| DOI: | 10.1038/nbt1132 |