دورية أكاديمية
Spatial expression of germ cell markers during maturation of human fetal male gonads: an immunohistochemical study.
| العنوان: | Spatial expression of germ cell markers during maturation of human fetal male gonads: an immunohistochemical study. |
|---|---|
| المؤلفون: | Pauls K; Institute of Pathology and Department of Developmental Pathology, University of Bonn, Germany. katrina.pauls@ukb.uni-bonn.de, Schorle H, Jeske W, Brehm R, Steger K, Wernert N, Büttner R, Zhou H |
| المصدر: | Human reproduction (Oxford, England) [Hum Reprod] 2006 Feb; Vol. 21 (2), pp. 397-404. Date of Electronic Publication: 2005 Oct 06. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Oxford University Press Country of Publication: England NLM ID: 8701199 Publication Model: Print-Electronic Cited Medium: Print ISSN: 0268-1161 (Print) Linking ISSN: 02681161 NLM ISO Abbreviation: Hum Reprod Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Oxford, UK : Oxford University Press Original Publication: Oxford ; Washington, DC : Published for the European Society of Human Reproduction and Embryology by IRL Press, [c1986- |
| مواضيع طبية MeSH: | Fetus/*metabolism , Germ Cells/*growth & development , Testis/*embryology, Antigens, Neoplasm/analysis ; Antigens, Neoplasm/metabolism ; Biomarkers/metabolism ; Biomarkers, Tumor/metabolism ; Cell Count ; Cell Differentiation ; Cell Proliferation ; Germ Cells/cytology ; Germ Cells/metabolism ; Humans ; Immunohistochemistry ; Male ; Neoplasm Proteins/metabolism ; Neoplasms, Germ Cell and Embryonal/metabolism ; Octamer Transcription Factor-3/metabolism ; Phenotype ; Proto-Oncogene Proteins c-kit/metabolism ; Sertoli Cells/metabolism ; Testis/immunology ; Testis/metabolism ; Transcription Factor AP-2/metabolism |
| مستخلص: | Background: The aim of the present study was to examine fetal male germ cells for expression of proteins associated with differentiation and maturation and to compare them with morphologically defined subpopulations. Methods: Testes of 61 fetuses from week 12 of gestation to the newborn period were selected. Immunohistochemistry was performed using antibodies to proteins associated with differentiation of germ cells (c-KIT, AP-2gamma) or pluripotency (OCT3/4), oncofetal protein M2A and spermatogonial marker MAGE-A4. Results: Two subtypes of fetal germ cells were detected by quantification and immunohistochemistry. Nearly all germ cells with morphological criteria of gonocytes and intermediate cells co-expressed OCT3/4, c-KIT, M2A and AP-2gamma. Starting from week 12, their number increased up to week 18/19 and then declined continuously during further development. After week 25, pre-spermatogonia were predominant and expressed MAGE-A4 selectively. Conclusions: Fetal male germ cells are comprised of two major groups with distinct immunohistochemical phenotypes. Germ cells that are predominantly found before week 25 of gestation co-express oncofetal proteins OCT3/4, c-KIT, M2A and AP-2gamma. After week 25, most germ cells have lost their pluripotent potential and acquire a spermatogonial phenotype defined by expression of MAGE-A4. |
| المشرفين على المادة: | 0 (Antigens, Neoplasm) 0 (Biomarkers) 0 (Biomarkers, Tumor) 0 (MAGEA4 protein, human) 0 (Neoplasm Proteins) 0 (Octamer Transcription Factor-3) 0 (Transcription Factor AP-2) 0 (oncofetal antigens) EC 2.7.10.1 (Proto-Oncogene Proteins c-kit) |
| تواريخ الأحداث: | Date Created: 20051008 Date Completed: 20060308 Latest Revision: 20220317 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1093/humrep/dei325 |
| PMID: | 16210381 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 0268-1161 |
|---|---|
| DOI: | 10.1093/humrep/dei325 |