دورية أكاديمية
أعرض في EDS

Binding of herpes simplex virus-1 US11 to specific RNA sequences.

التفاصيل البيبلوغرافية
العنوان: Binding of herpes simplex virus-1 US11 to specific RNA sequences.
المؤلفون: Bryant KF; Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, 250 Longwood Avenue, Boston, MA 02115, USA., Cox JC, Wang H, Hogle JM, Ellington AD, Coen DM
المصدر: Nucleic acids research [Nucleic Acids Res] 2005 Oct 24; Vol. 33 (19), pp. 6090-100. Date of Electronic Publication: 2005 Oct 24 (Print Publication: 2005).
نوع المنشور: Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, P.H.S.
اللغة: English
بيانات الدورية: Publisher: Oxford University Press Country of Publication: England NLM ID: 0411011 Publication Model: Electronic-Print Cited Medium: Internet ISSN: 1362-4962 (Electronic) Linking ISSN: 03051048 NLM ISO Abbreviation: Nucleic Acids Res Subsets: MEDLINE
أسماء مطبوعة: Publication: 1992- : Oxford : Oxford University Press
Original Publication: London, Information Retrieval ltd.
مواضيع طبية MeSH: Herpesvirus 1, Human*, RNA/*chemistry , RNA-Binding Proteins/*metabolism , Viral Proteins/*metabolism, Base Sequence ; Binding Sites ; Consensus Sequence ; Electrophoretic Mobility Shift Assay ; Hydroxyl Radical/chemistry ; Molecular Sequence Data ; Oligonucleotides/chemistry ; Protein Structure, Tertiary ; RNA/metabolism ; RNA, Double-Stranded/chemistry ; RNA, Double-Stranded/metabolism ; RNA-Binding Proteins/chemistry ; Ribonucleases/metabolism ; Viral Proteins/chemistry
مستخلص: Herpes simplex virus-1 US11 is a RNA-binding protein with a novel RNA-binding domain. US11 has been reported to exhibit sequence- and conformation-specific RNA-binding, but the sequences and conformations important for binding are not known. US11 has also been described as a double-stranded RNA (dsRNA)-binding protein. To investigate the US11-RNA interaction, we performed in vitro selection of RNA aptamers that bind US11 from a RNA library consisting of >10(14) 80 base sequences which differ in a 30 base randomized region. US11 bound specifically to selected aptamers with an affinity of 70 nM. Analysis of 23 selected sequences revealed a strong consensus sequence. The US11 RNA-binding domain and < or =46 bases of selected RNA containing the consensus sequence were each sufficient for binding. US11 binding protected the consensus motif from hydroxyl radical cleavage. RNase digestions of a selected aptamer revealed regions of both single-stranded RNA and dsRNA. We observed that US11 bound two different dsRNAs in a sequence non-specific manner, but with lower affinity than it bound selected aptamers. The results define a relatively short specific sequence that binds US11 with high affinity and indicate that dsRNA alone does not confer high-affinity binding.
References: J Virol. 1990 Jul;64(7):3463-70. (PMID: 2161949)
J Virol. 1991 Nov;65(11):5873-9. (PMID: 1656075)
J Virol. 1992 Jun;66(6):3624-32. (PMID: 1316472)
J Gen Virol. 1993 Mar;74 ( Pt 3):397-406. (PMID: 8383175)
Virology. 1993 May;194(1):419-23. (PMID: 8386887)
J Virol. 1995 Mar;69(3):1389-99. (PMID: 7853471)
Nature. 1996 Jan 18;379(6562):273-7. (PMID: 8538795)
J Virol. 1996 May;70(5):2842-51. (PMID: 8627758)
EMBO J. 1996 Sep 2;15(17):4759-66. (PMID: 8887567)
Cell Stress Chaperones. 1997 Jun;2(2):119-31. (PMID: 9250403)
J Biol Chem. 1998 Jun 5;273(23):14309-14. (PMID: 9603938)
J Gen Virol. 1998 Jul;79 ( Pt 7):1593-602. (PMID: 9680120)
J Virol. 1998 Nov;72(11):8620-6. (PMID: 9765401)
Bioorg Med Chem. 2001 Oct;9(10):2525-31. (PMID: 11557339)
Mol Cell. 2000 Feb;5(2):267-78. (PMID: 10882068)
J Virol. 1999 Jan;73(1):55-66. (PMID: 9847307)
J Virol. 2002 Apr;76(7):3282-91. (PMID: 11884553)
Comb Chem High Throughput Screen. 2002 Jun;5(4):289-99. (PMID: 12052180)
J Virol. 2002 Aug;76(16):8090-100. (PMID: 12134014)
J Virol. 2002 Dec;76(23):11971-81. (PMID: 12414939)
J Virol. 2003 Jan;77(1):228-36. (PMID: 12477828)
J Virol. 2003 Oct;77(20):10917-28. (PMID: 14512542)
معلومات مُعتمدة: R01 AI036083 United States AI NIAID NIH HHS; AI36083-10 United States AI NIAID NIH HHS; R01 AI019838 United States AI NIAID NIH HHS; AI19838 United States AI NIAID NIH HHS; T32 CA09031 United States CA NCI NIH HHS; T32 CA009031 United States CA NCI NIH HHS; R56 AI019838 United States AI NIAID NIH HHS
المشرفين على المادة: 0 (Oligonucleotides)
0 (RNA, Double-Stranded)
0 (RNA-Binding Proteins)
0 (US11 protein, herpesvirus)
0 (Viral Proteins)
3352-57-6 (Hydroxyl Radical)
63231-63-0 (RNA)
EC 3.1.- (Ribonucleases)
تواريخ الأحداث: Date Created: 20051026 Date Completed: 20051031 Latest Revision: 20240526
رمز التحديث: 20240526
مُعرف محوري في PubMed: PMC1266072
DOI: 10.1093/nar/gki919
PMID: 16246910
قاعدة البيانات: MEDLINE
الوصف
تدمد:1362-4962
DOI:10.1093/nar/gki919