دورية أكاديمية
Binding of herpes simplex virus-1 US11 to specific RNA sequences.
العنوان: | Binding of herpes simplex virus-1 US11 to specific RNA sequences. |
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المؤلفون: | Bryant KF; Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, 250 Longwood Avenue, Boston, MA 02115, USA., Cox JC, Wang H, Hogle JM, Ellington AD, Coen DM |
المصدر: | Nucleic acids research [Nucleic Acids Res] 2005 Oct 24; Vol. 33 (19), pp. 6090-100. Date of Electronic Publication: 2005 Oct 24 (Print Publication: 2005). |
نوع المنشور: | Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, P.H.S. |
اللغة: | English |
بيانات الدورية: | Publisher: Oxford University Press Country of Publication: England NLM ID: 0411011 Publication Model: Electronic-Print Cited Medium: Internet ISSN: 1362-4962 (Electronic) Linking ISSN: 03051048 NLM ISO Abbreviation: Nucleic Acids Res Subsets: MEDLINE |
أسماء مطبوعة: | Publication: 1992- : Oxford : Oxford University Press Original Publication: London, Information Retrieval ltd. |
مواضيع طبية MeSH: | Herpesvirus 1, Human*, RNA/*chemistry , RNA-Binding Proteins/*metabolism , Viral Proteins/*metabolism, Base Sequence ; Binding Sites ; Consensus Sequence ; Electrophoretic Mobility Shift Assay ; Hydroxyl Radical/chemistry ; Molecular Sequence Data ; Oligonucleotides/chemistry ; Protein Structure, Tertiary ; RNA/metabolism ; RNA, Double-Stranded/chemistry ; RNA, Double-Stranded/metabolism ; RNA-Binding Proteins/chemistry ; Ribonucleases/metabolism ; Viral Proteins/chemistry |
مستخلص: | Herpes simplex virus-1 US11 is a RNA-binding protein with a novel RNA-binding domain. US11 has been reported to exhibit sequence- and conformation-specific RNA-binding, but the sequences and conformations important for binding are not known. US11 has also been described as a double-stranded RNA (dsRNA)-binding protein. To investigate the US11-RNA interaction, we performed in vitro selection of RNA aptamers that bind US11 from a RNA library consisting of >10(14) 80 base sequences which differ in a 30 base randomized region. US11 bound specifically to selected aptamers with an affinity of 70 nM. Analysis of 23 selected sequences revealed a strong consensus sequence. The US11 RNA-binding domain and < or =46 bases of selected RNA containing the consensus sequence were each sufficient for binding. US11 binding protected the consensus motif from hydroxyl radical cleavage. RNase digestions of a selected aptamer revealed regions of both single-stranded RNA and dsRNA. We observed that US11 bound two different dsRNAs in a sequence non-specific manner, but with lower affinity than it bound selected aptamers. The results define a relatively short specific sequence that binds US11 with high affinity and indicate that dsRNA alone does not confer high-affinity binding. |
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معلومات مُعتمدة: | R01 AI036083 United States AI NIAID NIH HHS; AI36083-10 United States AI NIAID NIH HHS; R01 AI019838 United States AI NIAID NIH HHS; AI19838 United States AI NIAID NIH HHS; T32 CA09031 United States CA NCI NIH HHS; T32 CA009031 United States CA NCI NIH HHS; R56 AI019838 United States AI NIAID NIH HHS |
المشرفين على المادة: | 0 (Oligonucleotides) 0 (RNA, Double-Stranded) 0 (RNA-Binding Proteins) 0 (US11 protein, herpesvirus) 0 (Viral Proteins) 3352-57-6 (Hydroxyl Radical) 63231-63-0 (RNA) EC 3.1.- (Ribonucleases) |
تواريخ الأحداث: | Date Created: 20051026 Date Completed: 20051031 Latest Revision: 20240526 |
رمز التحديث: | 20240526 |
مُعرف محوري في PubMed: | PMC1266072 |
DOI: | 10.1093/nar/gki919 |
PMID: | 16246910 |
قاعدة البيانات: | MEDLINE |
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