دورية أكاديمية
[Influence of Qingdai compound on expression of bcr/abl and JWA in K562 cells].
| العنوان: | [Influence of Qingdai compound on expression of bcr/abl and JWA in K562 cells]. |
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| المؤلفون: | Dai HP; Department of Hematology, The Jiangsu TCM Hospital, Nanjing TCM University, Nanjing 210029, China., Shen Q, Zhou JW, Tang WY, Zhu GR, Xia W |
| المصدر: | Zhongguo shi yan xue ye xue za zhi [Zhongguo Shi Yan Xue Ye Xue Za Zhi] 2005 Oct; Vol. 13 (5), pp. 809-11. |
| نوع المنشور: | Journal Article |
| اللغة: | Chinese |
| بيانات الدورية: | Publisher: Zhongguo shi yan xue za zhi she Country of Publication: China NLM ID: 101084424 Publication Model: Print Cited Medium: Print ISSN: 1009-2137 (Print) Linking ISSN: 10092137 NLM ISO Abbreviation: Zhongguo Shi Yan Xue Ye Xue Za Zhi Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Beijing : Zhongguo shi yan xue za zhi she, |
| مواضيع طبية MeSH: | Apoptosis/*drug effects , Drugs, Chinese Herbal/*pharmacology , Fusion Proteins, bcr-abl/*genetics , Heat-Shock Proteins/*genetics , Intracellular Signaling Peptides and Proteins/*genetics, Antineoplastic Agents, Phytogenic/pharmacology ; Cell Proliferation/drug effects ; Dose-Response Relationship, Drug ; Gene Expression Regulation, Neoplastic/drug effects ; Humans ; K562 Cells ; Membrane Transport Proteins ; RNA, Messenger/biosynthesis ; RNA, Messenger/genetics ; Reverse Transcriptase Polymerase Chain Reaction |
| مستخلص: | To study the effects of Qingdai compound on proliferation and apoptosis of K562 cells, as well as the expression of bcr/abl and JWA mRNA, K562 cells were treated in culture with different concentrations of Qingdai compound (2.5, 5, 7.5, 10 and 20 mg/ml) and harvested at 24 hours. Then morphological changes were observed by light microscopy (LM); expressions of bcr/abl and JWA were detected with semi-quantitative RT-PCR. The results showed that morphological changes were observed as the increment of the Qingdai compound concentration. Inhibition effects on proliferation and apoptosis in K562 cells were seen. A concentration-dependent decreases were found in bcr-abl and JWA mRNA expression of K562 cells. Qingdai compound partially inhibited proliferation and induced apoptosis of K562 cells. Expressions of both bcr/abl and JWA, which took part in cell proliferation and apoptosis, were down-regulated in a dose dependent manner. In conclusion, Qingdai compound can partially inhibit the expressions of bcr/abl and JWA genes in K562 cells, and the clinical effect of Qingdai compound on CML may be associated with apoptosis of leukemic cells. |
| المشرفين على المادة: | 0 (ARL6IP5 protein, human) 0 (Antineoplastic Agents, Phytogenic) 0 (Drugs, Chinese Herbal) 0 (Heat-Shock Proteins) 0 (Intracellular Signaling Peptides and Proteins) 0 (Membrane Transport Proteins) 0 (RNA, Messenger) EC 2.7.10.2 (Fusion Proteins, bcr-abl) R1R8QUL21Z (Qingdai compound) |
| تواريخ الأحداث: | Date Created: 20051110 Date Completed: 20080905 Latest Revision: 20191210 |
| رمز التحديث: | 20221213 |
| PMID: | 16277847 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1009-2137 |
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