دورية أكاديمية
St. Louis encephalitis virus: first isolation from a human in São Paulo State, Brazil.
| العنوان: | St. Louis encephalitis virus: first isolation from a human in São Paulo State, Brazil. |
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| المؤلفون: | Rocco IM; Instituto Adolfo Lutz, São Paulo, SP, Brazil. imrocco@ial.sp.gov.br, Santos CL, Bisordi I, Petrella SM, Pereira LE, Souza RP, Coimbra TL, Bessa TA, Oshiro FM, Lima LB, Cerroni MP, Marti AT, Barbosa VM, Katz G, Suzuki A |
| المصدر: | Revista do Instituto de Medicina Tropical de Sao Paulo [Rev Inst Med Trop Sao Paulo] 2005 Sep-Oct; Vol. 47 (5), pp. 281-5. Date of Electronic Publication: 2005 Nov 16. |
| نوع المنشور: | Case Reports; Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Universidade de São Paulo, Instituto de Medicina Tropical de São Paulo Country of Publication: Brazil NLM ID: 7507484 Publication Model: Print-Electronic Cited Medium: Print ISSN: 0036-4665 (Print) Linking ISSN: 00364665 NLM ISO Abbreviation: Rev Inst Med Trop Sao Paulo Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Sao Paulo : Universidade de São Paulo, Instituto de Medicina Tropical de São Paulo |
| مواضيع طبية MeSH: | Antibodies, Viral/*blood , Encephalitis Virus, St. Louis/*isolation & purification , Encephalitis, St. Louis/*diagnosis, Brazil ; Encephalitis Virus, St. Louis/genetics ; Encephalitis Virus, St. Louis/immunology ; Enzyme-Linked Immunosorbent Assay/methods ; Female ; Humans ; Immunoglobulin G/blood ; Middle Aged ; RNA, Viral/analysis ; Reverse Transcriptase Polymerase Chain Reaction |
| مستخلص: | This paper reports the isolation of St. Louis encephalitis virus (SLEV) from a febrile human case suspected to be dengue, in São Pedro, São Paulo State. A MAC-ELISA done on the patient's acute and convalescent sera was inconclusive and hemagglutination inhibition test detected IgG antibody for flaviviruses. An indirect immunofluorescent assay done on the C6/36 cell culture inoculated with the acute serum was positive for flaviviruses but negative when tested with dengue monoclonal antibodies. RNA extracted from the infected cell culture supernatant was amplified by RT-PCR in the presence of NS5 universal flavivirus primers and directly sequenced. Results of BLAST search indicated that this sequence shares 93% nucleotide similarity with the sequence of SLEV (strain-MSI.7), confirmed by RT-PCR performed with SLEV specific primers. Since SLEV was identified as the cause of human disease, it is necessary to improve surveillance in order to achieve early detection of this agent in the state of São Paulo and in Brazil. This finding is also an alert to health professionals about the need for more complete clinical and epidemiological investigations of febrile illnesses as in the reported case. SLEV infections can be unrecognized or confused with other ones caused by an arbovirus, such as dengue. |
| المشرفين على المادة: | 0 (Antibodies, Viral) 0 (Immunoglobulin G) 0 (RNA, Viral) |
| تواريخ الأحداث: | Date Created: 20051123 Date Completed: 20060124 Latest Revision: 20190608 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1590/s0036-46652005000500008 |
| PMID: | 16302112 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 0036-4665 |
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| DOI: | 10.1590/s0036-46652005000500008 |