دورية أكاديمية
Polyvinylpyrrolidone-agarose gel electrophoresis purification of polymerase chain reaction-amplifiable DNA from soils.
| العنوان: | Polyvinylpyrrolidone-agarose gel electrophoresis purification of polymerase chain reaction-amplifiable DNA from soils. |
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| المؤلفون: | Young CC; Exxon Research and Engineering Company, Route 22 East, Annandale, New Jersey 08801., Burghoff RL, Keim LG, Minak-Bernero V, Lute JR, Hinton SM |
| المصدر: | Applied and environmental microbiology [Appl Environ Microbiol] 1993 Jun; Vol. 59 (6), pp. 1972-4. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Society for Microbiology Country of Publication: United States NLM ID: 7605801 Publication Model: Print Cited Medium: Print ISSN: 0099-2240 (Print) Linking ISSN: 00992240 NLM ISO Abbreviation: Appl Environ Microbiol Subsets: PubMed not MEDLINE |
| أسماء مطبوعة: | Original Publication: Washington, American Society for Microbiology. |
| مستخلص: | This communication describes a modification of agarose gel electrophoresis to provide a rapid and simple method for the purification of polymerase chain reaction-amplifiable DNA from soil. This modification is to add polyvinylpyrrolidone to the agarose gel. The polyvinylpyrrolidone addition retards the electrophoretic mobility of denaturing phenolic compounds so that they do not comigrate with nucleic acids. |
| References: | Appl Environ Microbiol. 1988 Apr;54(4):1070. (PMID: 16347606) Appl Environ Microbiol. 1992 Feb;58(2):754-7. (PMID: 1610201) Nucleic Acids Res. 1992 May 11;20(9):2381. (PMID: 1375739) Appl Environ Microbiol. 1988 Mar;54(3):703-711. (PMID: 16347582) Proc Natl Acad Sci U S A. 1985 Oct;82(20):6955-9. (PMID: 2413450) Appl Environ Microbiol. 1991 Apr;57(4):1070-4. (PMID: 1647749) |
| تواريخ الأحداث: | Date Created: 19930601 Date Completed: 20100707 Latest Revision: 20210526 |
| رمز التحديث: | 20231215 |
| مُعرف محوري في PubMed: | PMC182198 |
| DOI: | 10.1128/aem.59.6.1972-1974.1993 |
| PMID: | 16348974 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 0099-2240 |
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| DOI: | 10.1128/aem.59.6.1972-1974.1993 |