دورية أكاديمية
Characterization of a muscle-specific enhancer in human MuSK promoter reveals the essential role of myogenin in controlling activity-dependent gene regulation.
| العنوان: | Characterization of a muscle-specific enhancer in human MuSK promoter reveals the essential role of myogenin in controlling activity-dependent gene regulation. |
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| المؤلفون: | Tang H; Molecular and Behavior Neuroscience Institute and Department of Biological Chemistry, University of Michigan, Ann Arbor, 48109, USA., Veldman MB, Goldman D |
| المصدر: | The Journal of biological chemistry [J Biol Chem] 2006 Feb 17; Vol. 281 (7), pp. 3943-53. Date of Electronic Publication: 2005 Dec 18. |
| نوع المنشور: | Journal Article; Research Support, N.I.H., Extramural |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Inc. on behalf of American Society for Biochemistry and Molecular Biology Country of Publication: United States NLM ID: 2985121R Publication Model: Print-Electronic Cited Medium: Print ISSN: 0021-9258 (Print) Linking ISSN: 00219258 NLM ISO Abbreviation: J Biol Chem Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2021- : [New York, NY] : Elsevier Inc. on behalf of American Society for Biochemistry and Molecular Biology Original Publication: Baltimore, MD : American Society for Biochemistry and Molecular Biology |
| مواضيع طبية MeSH: | Gene Expression Regulation* , Promoter Regions, Genetic*, Enhancer Elements, Genetic/*physiology , Muscle, Skeletal/*metabolism , Myogenin/*physiology , Receptor Protein-Tyrosine Kinases/*genetics , Receptors, Cholinergic/*genetics, Animals ; Base Sequence ; Calcium/physiology ; Cell Differentiation ; Cells, Cultured ; Humans ; Molecular Sequence Data ; Receptors, Nicotinic/genetics ; Transcriptional Activation ; Zebrafish/metabolism |
| مستخلص: | Neuromuscular synaptogenesis is initiated by the release of agrin from motor neurons and the activation of the receptor tyrosine kinase, MuSK, in the postsynaptic membrane. MuSK gene expression is regulated by nerve-derived agrin and muscle activity. Agrin stimulates synapse-specific MuSK gene expression by activating GABP(alphabeta) transcription factors in endplate-associated myonuclei. In contrast, the mechanism by which muscle activity regulates MuSK gene expression is not known. We report on a 60-bp MuSK enhancer that confers promoter regulation by muscle differentiation, changes in intracellular calcium, and muscle activity. Within this enhancer, we identified a single E-box that is essential for this regulation. This E-box binds myogenin, and we showed that myogenin is necessary for not only MuSK but also nAChR gene regulation by muscle activity. Surprisingly, the same E-box functions in vivo to mediate muscle-specific and differentiation-dependent gene induction in zebrafish, suggesting an evolutionary conserved mechanism of regulation of synaptic protein gene expression. |
| معلومات مُعتمدة: | R01 NS25153 United States NS NINDS NIH HHS |
| المشرفين على المادة: | 0 (Myogenin) 0 (Receptors, Cholinergic) 0 (Receptors, Nicotinic) EC 2.7.10.1 (MUSK protein, human) EC 2.7.10.1 (Receptor Protein-Tyrosine Kinases) SY7Q814VUP (Calcium) |
| تواريخ الأحداث: | Date Created: 20051220 Date Completed: 20060328 Latest Revision: 20220309 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1074/jbc.M511317200 |
| PMID: | 16361705 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 0021-9258 |
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| DOI: | 10.1074/jbc.M511317200 |