دورية أكاديمية
Prognostic value of canine frozen-thawed semen parameters on in vitro sperm-oocyte interactions.
| العنوان: | Prognostic value of canine frozen-thawed semen parameters on in vitro sperm-oocyte interactions. |
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| المؤلفون: | Silva AR; Laboratory of Carnivore Reproduction-FAVET, UECE, Paranjana Ave. 1700, Itaperi 60740-000, Fortaleza, Ceará, Brazil. legio2000@yahoo.com, Cardoso Rde C, Silva LD, Chirinéa VH, Lopes MD, Souza FF |
| المصدر: | Theriogenology [Theriogenology] 2006 Jul 15; Vol. 66 (2), pp. 456-62. Date of Electronic Publication: 2006 Jan 19. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: United States NLM ID: 0421510 Publication Model: Print-Electronic Cited Medium: Print ISSN: 0093-691X (Print) Linking ISSN: 0093691X NLM ISO Abbreviation: Theriogenology Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: [New York, N.Y.?] : Elsevier Original Publication: Los Altos, Calif., Geron-X. |
| مواضيع طبية MeSH: | Cryopreservation/*veterinary , Dogs/*physiology , Semen Preservation/*veterinary , Sperm-Ovum Interactions/*physiology , Spermatozoa/*physiology, Animals ; Cell Membrane/physiology ; Cryopreservation/methods ; Ejaculation ; Female ; Image Processing, Computer-Assisted ; Linear Models ; Male ; Oocytes/physiology ; Predictive Value of Tests ; Semen Preservation/methods ; Sperm Capacitation/physiology ; Sperm Motility |
| مستخلص: | Combining the data from conventional semen analysis with oocyte penetration assays should improve the assessment of the fertilizing ability of a semen sample. Thus, the objective of the present study was to evaluate the prognostic value of various semen parameters on the in vitro interactions between frozen-thawed canine sperm and homologous oocytes. Ten ejaculates from five stud dogs (two ejaculates/dog) were collected by digital manipulation. Semen samples were evaluated, extended in Tris-egg yolk-glycerol, frozen and stored in liquid nitrogen, and thawed several weeks later. Samples were evaluated for motility and sperm populations by computer-aided semen analysis (CASA), plasma membrane integrity (carboxy-fluorescein diacetate and propidium iodide), and sperm morphology (Bengal Rose). Thawed spermatozoa were also incubated with homologous oocytes for 18 h in an atmosphere of 5% CO(2) and 95% air at 38 degrees C and sperm-oocyte interactions were evaluated. Simple linear regression models were calculated, with sperm parameters as independent variables and sperm-oocyte interactions as the dependent variable. There were significant associations between: percentage of oocytes bound to spermatozoa and beat cross frequency (BCF; R(2)=63%); percentage of oocytes that interacted with spermatozoa and BCF (R(2)=73%); and number of penetrated spermatozoa and velocity average pathway (VAP; R(2)=64%) and velocity straight line (VSL; R(2)=64%). Although plasma membrane integrity and sperm morphology had little prognostic value for in vitro interactions between canine frozen-thawed sperm and homologous oocytes, some motility patterns (evaluated by CASA) were predictive of in vitro sperm-oocyte interactions. |
| تواريخ الأحداث: | Date Created: 20060124 Date Completed: 20070712 Latest Revision: 20060626 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1016/j.theriogenology.2005.12.009 |
| PMID: | 16427120 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 0093-691X |
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| DOI: | 10.1016/j.theriogenology.2005.12.009 |